Lipid metabolism and m6A RNA methylation are altered in lambs supplemented rumen-protected methionine and lysine in a low-protein diet

Table 4 Effect of a low-protein diet supplemented with rumen-protected methionine and lysine on the fatty acids composition of liver in growing lambs, % of total fatty acids

Fatty acids, %	Treatment			SEM	P-value
Fatty acids, %	NP	LP	LP + RML	SEM	P-value
C12:0	0.239	0.244	0.256	0.012	0.563
C14:0	2.625	2.862	2.885	0.128	0.318
C14:1	0.169	0.168	0.164	0.021	0.883
C15:0	0.277	0.329	0.316	0.015	0.062
C16:0	23.84^b	26.08^a	24.86^ab	0.561	0.040
C16:1	1.552^b	2.086^a	2.119^a	0.138	0.020
C17:0	0.992	1.049	1.014	0.075	0.875
C18:0	15.649	15.21	15.28	0.502	0.035
C18:1n-9 t	0.358^b	0.423^a	0.356^b	0.015	0.011
C18:1n-9c	41.619	40.608	40.989	0.548	0.441
C18:2n-6c	8.496^a	7.204^b	7.787^ab	0.288	0.029
C20:0	0.159	0.158	0.173	0.018	0.861
C18:3n-3	2.465^a	2.173^b	2.194^b	0.071	0.019
C20:3n-6	0.323^a	0.233^b	0.231^b	0.019	0.005
C20:4n-6	0.325	0.296	0.305	0.032	0.861
C24:0	0.544	0.587	0.606	0.051	0.699
C22:6n-3	0.368	0.297	0.362	0.043	0.533
Fatty acid partial sums
SFA¹	44.32^b	46.52^a	45.40^ab	0.433	0.009
MUFA²	43.69	43.28	43.63	0.551	0.721
PUFA³	11.98^a	10.20^b	10.97^ab	0.308	0.007
∑n-6 PUFA⁴	9.14^a	7.73^b	8.41^ab	0.276	0.018
∑n-3 PUFA⁵	2.83^a	2.47^b	2.56^b	0.069	0.005

Different letters across a row indicate a significant difference among the three dietary treatments (P < 0.05). Data are presented as means with their pooled SEM
NP adequate protein diet (n = 7), LP low-protein diet (n = 6), LP + RML low-protein diet supplemented with rumen-protected methionine and lysine (n = 7)
¹SFA (saturated fatty acid) = C12:0 + C14:0 + C15:0 + C16:0 + C17:0 + C18:0 + C20:0 + C24:0
²MUFA (monounsaturated fatty acid) = C14:1 + C16:1 + C18:1n-9 t + C18:1n-9c
³PUFA (polyunsaturated fatty acid) = C18:2n-6c + C18:3n-3 + C20:3n-6 + C20:4n-6 + C22:6n-3
⁴n-3 PUFA = C18:3n-3 + C22:6n-3
⁵n-6 PUFA = C18:2n-6c + C20:3n-6 + C20:4n-6

ISSN: 2049-1891