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Table 9 Effect of the dietary arginine level in a low-protein diet on intestinal morphology1

From: The optimal dietary arginine level of laying hens fed with low-protein diets

Item

Control2

Dietary arginine level in LP diet, %3

P-value

0.80

0.85

0.90

0.95

1.00

Diet4

Linear5

Quadratic5

Duodenum

 Villus height, μm

1854 ± 84a

1612 ± 47bc

1735 ± 35ab

1723 ± 26ab

1577 ± 62bc

1498 ± 71c

0.001

0.011

0.003

 Crypt depth, μm

275.9 ± 26.0

269.2 ± 18.1

248.7 ± 12.0

304.9 ± 19.8

293.6 ± 9.6

290.7 ± 11.8

0.255

0.069

0.561

 Villus height:crypt depth

7.17 ± 0.71a

6.17 ± 0.42ab

7.04 ± 0.22a

5.83 ± 0.40ab

5.43 ± 0.32b

5.25 ± 0.39b

0.011

0.004

0.318

Jejunum

 Villus height, μm

1574 ± 74a

1493 ± 34ab

1646 ± 43a

1544 ± 53ab

1394 ± 65bc

1313 ± 47c

0.001

< 0.001

0.004

 Crypt depth, μm

225.9 ± 5.0

257.5 ± 24.2

236.0 ± 12.7

247.4 ± 12.8

223.1 ± 13.0

232.2 ± 8.9

0.499

0.166

0.654

 Villus height:crypt depth

7.01 ± 0.40a

6.08 ± 0.47ab

7.08 ± 0.36a

6.35 ± 0.37ab

6.30 ± 0.22ab

5.69 ± 0.23b

0.012

0.155

0.048

Ileum

 Villus height, μm

1022 ± 48

1012 ± 52

1015 ± 25

965.1 ± 55.1

987.9 ± 26.1

930.3 ± 26.4

0.592

0.156

0.755

 Crypt depth, μm

157.3 ± 4.7

192.4 ± 14.4

176.7 ± 6.8

174.4 ± 10.1

159.9 ± 8.0

173.3 ± 10.4

0.140

0.096

0.236

 Villus height:crypt depth

6.51 ± 0.29

5.53 ± 0.59

5.80 ± 0.25

5.65 ± 0.39

6.28 ± 0.34

5.51 ± 0.37

0.340

0.729

0.385

  1. 1Data were presented as the mean ± SD (n = 8)
  2. 2The control hens were fed a diet with 16% protein level
  3. 3The experimental groups were provided a 14% protein diets (0.80% Arginine) supplemented with 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg
  4. 4Diet = effect of all the dietary treatments
  5. 5Linear and quadratic = effect of dietary arginine from 0.80% to 1.00% treatments
  6. a-c: Means with different superscripts within the same column differ significantly, P < 0.05