The optimal dietary arginine level of laying hens fed with low-protein diets

Table 8 Effect of the dietary arginine level in a low-protein diet on anti-oxidative parameters in tissues¹

Item, nmol/mg prot	Control²	Dietary arginine level in LP diet, %³					P-value
		0.80	0.85	0.90	0.95	1.00	P-value
		0.80	0.85	0.90	0.95	1.00	Diet⁴	Linear⁵	Quadratic⁵
Liver
MDA	0.33 ± 0.03	0.35 ± 0.05	0.38 ± 0.03	0.36 ± 0.03	0.36 ± 0.04	0.40 ± 0.03	0.772	0.428	0.836
Protein carbonyl	1.56 ± 0.07	1.55 ± 0.14	1.27 ± 0.04	1.32 ± 0.07	1.72 ± 0.22	1.31 ± 0.08	0.059	0.619	0.747
T-AOC	78.9 ± 4.31^a	74.6 ± 3.39^ab	73.5 ± 4.81^ab	72.0 ± 4.09^abc	65.5 ± 3.28^bc	61.0 ± 2.30^c	0.022	0.003	0.370
Duodenum
MDA	1.08 ± 0.16^ab	0.73 ± 0.07^b	0.60 ± 0.11^b	1.20 ± 0.23^ab	1.25 ± 0.14^ab	1.62 ± 0.42^a	0.027	0.002	0.592
Protein carbonyl	1.91 ± 0.09	1.88 ± 0.44	1.96 ± 0.23	1.98 ± 0.18	2.12 ± 0.16	1.67 ± 0.18	0.885	0.814	0.347
T-AOC	169.5 ± 6.6^bcd	188.4 ± 6.3^ab	195.0 ± 10.8^a	180.1 ± 6.5^abc	164.7 ± 6.0^cd	150.6 ± 5.7^d	< 0.001	< 0.001	0.127
Jejunum
MDA	0.27 ± 0.05^b	0.34 ± 0.08^ab	0.22 ± 0.03^b	0.34 ± 0.03^ab	0.37 ± 0.05^ab	0.47 ± 0.07^a	0.044	0.032	0.108
Protein carbonyl	2.15 ± 0.58	2.87 ± 0.55	1.61 ± 0.32	2.43 ± 0.68	1.55 ± 0.32	2.81 ± 0.58	0.347	0.733	0.163
T-AOC	289.0 ± 17.7^a	274.4 ± 10.3^a	275.0 ± 11.7^a	262.6 ± 11.9^ab	269.2 ± 19.8^ab	222.1 ± 11.5^b	0.007	0.021	0.176
Ileum
MDA	0.56 ± 0.10	0.61 ± 0.13	0.43 ± 0.05	0.67 ± 0.10	0.60 ± 0.12	0.52 ± 0.06	0.603	0.924	0.837
Protein carbonyl	2.18 ± 0.29	1.91 ± 0.09	2.32 ± 0.20	2.48 ± 0.28	1.89 ± 0.21	2.31 ± 0.14	0.319	0.452	0.246
T-AOC	283.8 ± 11.5	290.7 ± 14.2	281.9 ± 5.2	270.7 ± 9.1	281.6 ± 4.2	255.9 ± 8.5	0.205	0.028	0.752

¹Data were presented as the mean ± SD (n = 8). Malondialdehyde (MDA) contents, Total antioxidant capacity (T-AOC)
²The control hens were fed a diet with 16% protein level
³The experimental groups were provided a 14% protein diets (0.80% Arginine) supplemented with 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg
⁴Diet = effect of all the dietary treatments
⁵Linear and quadratic = effect of dietary arginine from 0.80% to 1.00% treatments
^a-d: Means with different superscripts within the same column differ significantly, P < 0.05

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