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Table 7 Effect of the dietary arginine level in a low-protein diet on plasma free AA concentrations1

From: The optimal dietary arginine level of laying hens fed with low-protein diets

Amino acids, μg/mL

Control2

Dietary arginine level in LP diet, %3

P-value

0.80

0.85

0.90

0.95

1.00

Diet4

Linear5

Quadratic5

Indispensable AA

 Lys

63.69 ± 2.26

69.83 ± 5.02

79.15 ± 6.69

53.35 ± 6.69

66.08 ± 5.40

64.15 ± 4.53

0.075

0.286

0.500

 Met

9.65 ± 0.44

9.45 ± 0.80

10.53 ± 0.57

10.36 ± 0.83

9.82 ± 0.57

10.91 ± 0.44

0.578

0.385

0.722

 Arg

43.26 ± 1.74

42.91 ± 2.54

41.19 ± 3.01

50.57 ± 4.16

48.43 ± 2.00

44.61 ± 2.52

0.156

0.333

0.142

 Val

3.35 ± 0.70

2.97 ± 0.30

3.64 ± 0.36

2.11 ± 0.23

2.33 ± 0.29

2.88 ± 0.26

0.070

0.145

0.209

 Ile

9.83 ± 0.59

10.47 ± 0.75

10.87 ± 0.46

9.95 ± 0.86

9.86 ± 0.44

10.35 ± 0.70

0.836

0.562

0.708

 Leu

19.60 ± 1.02

20.88 ± 1.11

21.87 ± 0.54

23.21 ± 2.26

20.62 ± 0.78

21.08 ± 0.92

0.440

0.797

0.289

 Phe

13.18 ± 0.52

13.13 ± 0.35

13.58 ± 0.64

13.43 ± 0.58

13.83 ± 0.55

13.43 ± 0.26

0.929

0.557

0.604

 Thr

28.11 ± 3.53

28.17 ± 2.54

32.36 ± 3.12

25.60 ± 2.51

24.02 ± 2.90

28.04 ± 1.61

0.409

0.352

0.653

 His

14.95 ± 1.69b

18.29 ± 0.94b

22.16 ± 0.87a

17.39 ± 0.99b

18.04 ± 1.15b

16.98 ± 1.39b

0.005

0.070

0.320

Dispensable AA

 Gly

33.41 ± 2.23

33.77 ± 1.46

38.16 ± 1.86

33.99 ± 1.88

33.95 ± 1.95

31.88 ± 1.12

0.272

0.135

0.160

 Tau

37.81 ± 3.84

32.05 ± 2.64

34.15 ± 2.10

27.74 ± 2.53

34.43 ± 2.22

35.99 ± 3.28

0.195

0.353

0.224

 Asp

3.50 ± 0.62

2.65 ± 0.28

3.41 ± 0.49

3.84 ± 0.95

2.64 ± 0.66

2.00 ± 0.28

0.306

0.213

0.045

 Ser

57.62 ± 3.13b

63.40 ± 3.15ab

71.98 ± 2.96a

66.75 ± 3.34ab

59.02 ± 3.75b

58.29 ± 2.73b

0.017

0.046

0.116

 Ala

35.86 ± 2.59

34.80 ± 1.84

38.21 ± 1.30

34.35 ± 2.69

37.54 ± 3.90

37.97 ± 3.07

0.853

0.575

0.950

 Cit

1.50 ± 0.06

1.61 ± 0.06

1.58 ± 0.08

1.42 ± 0.06

1.59 ± 0.07

1.49 ± 0.06

0.256

0.352

0.395

 Cys

38.21 ± 4.14

38.74 ± 2.68

39.66 ± 1.71

36.82 ± 3.75

37.11 ± 1.78

38.81 ± 1.36

0.979

0.737

0.664

 Tyr

17.24 ± 0.65

18.12 ± 1.95

19.47 ± 1.16

15.76 ± 0.74

18.28 ± 1.18

18.25 ± 0.89

0.364

0.712

0.531

 Orn

6.97 ± 0.63

5.83 ± 0.66

7.01 ± 0.74

9.72 ± 1.93

6.59 ± 0.51

8.95 ± 0.90

0.080

0.116

0.341

 Indispensable AA

205.6 ± 7.0

216.1 ± 10.9

235.3 ± 11.2

206.0 ± 9.5

213.0 ± 9.2

218.1 ± 6.6

0.255

0.538

0.909

 Dispensable AA

232.1 ± 7.9

231.0 ± 7.1

253.6 ± 4.1

230.4 ± 10.4

231.2 ± 12.1

233.6 ± 7.6

0.365

0.465

0.549

 Total AA

437.7 ± 12.8

447.1 ± 14.3

489.0 ± 14.8

436.4 ± 17.3

444.2 ± 20.0

456.3 ± 10.8

0.176

0.492

0.857

  1. 1Data were presented as the mean ± SD (n = 8)
  2. 2The control hens were fed a diet with 16% protein level
  3. 3The experimental groups were provided a 14% protein diets (0.80% Arginine) supplemented with 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg
  4. 4Diet = effect of all the dietary treatments
  5. 5Linear and quadratic = effect of dietary arginine from 0.80% to 1.00% treatments
  6. a,b: Means with different superscripts within the same column differ significantly, P < 0.05