The optimal dietary arginine level of laying hens fed with low-protein diets

Sun, Mingfa; Ma, Ning; Liu, Hui; Liu, Yu; Zhou, Yunlei; Zhao, Jingpeng; Wang, Xiaojuan; Li, Haifang; Ma, Baishun; Jiao, Hongchao; Lin, Hai

doi:10.1186/s40104-022-00719-x

Journal of Animal Science and Biotechnology

Table 6 Effect of the dietary arginine level in a low-protein diet on plasma anti-oxidative parameters¹

From: The optimal dietary arginine level of laying hens fed with low-protein diets

Item	Control²	Dietary arginine level in LP diet, %³					P-value
		0.80	0.85	0.90	0.95	1.00	P-value
		0.80	0.85	0.90	0.95	1.00	Diet⁴	Linear⁵	Quadratic⁵
Week 4
MDA, nmol/mL	5.85 ± 0.54^b	5.67 ± 0.25^b	5.80 ± 0.24^b	6.00 ± 0.34^b	6.38 ± 0.54^ab	7.56 ± 0.48^a	0.022	< 0.001	0.092
T-AOC, mmol/L	0.26 ± 0.03	0.24 ± 0.03	0.24 ± 0.02	0.20 ± 0.03	0.18 ± 0.04	0.25 ± 0.02	0.435	0.814	0.373
NO, μmol/L	1.45 ± 0.23	1.35 ± 0.29	1.62 ± 0.21	1.21 ± 0.19	1.60 ± 0.26	1.70 ± 0.22	0.259	0.396	0.312
TNOS, U/mL	29.8 ± 1.54	28.0 ± 0.95	30.0 ± 1.50	29.3 ± 2.70	30.7 ± 1.69	32.3 ± 1.53	0.626	0.087	0.840
iNOS, U/mL	11.6 ± 1.28	12.2 ± 1.06	11.4 ± 0.82	9.78 ± 0.77	11.9 ± 1.26	13.2 ± 1.26	0.403	0.063	< 0.001
CAT, U/mL	4.62 ± 0.44^ab	1.97 ± 0.39^c	4.28 ± 0.91^ab	5.81 ± 0.81^a	2.93 ± 0.44^bc	1.30 ± 0.29^c	< 0.001	0.169	< 0.001
T-SOD, U/mL	119.8 ± 1.4	121.0 ± 0.7	121.5 ± 0.5	122.0 ± 0.7	121.6 ± 0.5	121.4 ± 0.8	0.539	0.691	0.375
GSH-PX, IU	1794 ± 102	1861 ± 68	1697 ± 76	1861 ± 53	1895 ± 94	1886 ± 71	0.484	0.391	0.812
Week 8
MDA, nmol/mL	4.73 ± 0.63^b	5.13 ± 0.53^b	5.05 ± 0.54^b	5.99 ± 0.44^ab	4.39 ± 0.51^b	6.91 ± 0.48^a	0.040	0.153	0.240
T-AOC, mmol/L	0.26 ± 0.02^a	0.15 ± 0.03^b	0.24 ± 0.05^ab	0.22 ± 0.03^ab	0.21 ± 0.02^ab	0.17 ± 0.04^ab	0.035	0.963	0.112
NO, μmol/L	5.51 ± 1.07	5.27 ± 0.97	7.60 ± 0.98	10.44 ± 2.29	7.78 ± 1.92	5.25 ± 0.61	0.226	0.975	0.009
TNOS, U/mL	38.8 ± 1.45	39.1 ± 0.78	38.5 ± 0.88	38.9 ± 1.48	33.3 ± 3.51	41.6 ± 1.24	0.075	0.974	0.110
iNOS, U/mL	9.19 ± 0.95	9.60 ± 1.20	9.43 ± 0.41	9.06 ± 1.42	9.28 ± 1.87	9.87 ± 0.70	0.997	0.928	0.680
CAT, U/mL	7.23 ± 1.62^a	3.21 ± 0.48^b	3.79 ± 0.72^b	3.18 ± 0.42^b	2.98 ± 0.50^b	2.45 ± 0.48^b	0.002	0.161	0.325
T-SOD, U/mL	149.0 ± 4.8	137.7 ± 3.5	138.5 ± 4.2	145.4 ± 4.4	139.4 ± 4.8	143.9 ± 3.7	0.370	0.318	0.665
GSH-PX, IU	2809 ± 149	3130 ± 212	2980 ± 149	2777 ± 118	2684 ± 96	2888 ± 82	0.306	0.124	0.198

¹Data were presented as the mean ± SD (n = 15). Malondialdehyde (MDA) contents, Total antioxidant capacity (T-AOC), Nitric oxide (NO), Total nitric oxide synthase (tNOS) enzyme activity, Inducible nitric oxide synthase (iNOS) enzyme activity, Catalase (CAT) activity, Total superoxide dismutase (T-SOD) activity, Glutathione peroxidase (GSH-PX)
²The control hens were fed a diet with 16% protein level
³The experimental groups were provided a 14% protein diets (0.80% Arginine) supplemented with 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg
⁴Diet = effect of all the dietary treatments
⁵Linear and quadratic = effect of dietary arginine from 0.80% to 1.00% treatments
^a-c: Means with different superscripts within the same column differ significantly, P < 0.05

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ISSN: 2049-1891

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