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Table 6 Effect of the dietary arginine level in a low-protein diet on plasma anti-oxidative parameters1

From: The optimal dietary arginine level of laying hens fed with low-protein diets

Item Control2 Dietary arginine level in LP diet, %3 P-value
0.80 0.85 0.90 0.95 1.00
Diet4 Linear5 Quadratic5
Week 4
 MDA, nmol/mL 5.85 ± 0.54b 5.67 ± 0.25b 5.80 ± 0.24b 6.00 ± 0.34b 6.38 ± 0.54ab 7.56 ± 0.48a 0.022 < 0.001 0.092
 T-AOC, mmol/L 0.26 ± 0.03 0.24 ± 0.03 0.24 ± 0.02 0.20 ± 0.03 0.18 ± 0.04 0.25 ± 0.02 0.435 0.814 0.373
 NO, μmol/L 1.45 ± 0.23 1.35 ± 0.29 1.62 ± 0.21 1.21 ± 0.19 1.60 ± 0.26 1.70 ± 0.22 0.259 0.396 0.312
 TNOS, U/mL 29.8 ± 1.54 28.0 ± 0.95 30.0 ± 1.50 29.3 ± 2.70 30.7 ± 1.69 32.3 ± 1.53 0.626 0.087 0.840
 iNOS, U/mL 11.6 ± 1.28 12.2 ± 1.06 11.4 ± 0.82 9.78 ± 0.77 11.9 ± 1.26 13.2 ± 1.26 0.403 0.063 < 0.001
 CAT, U/mL 4.62 ± 0.44ab 1.97 ± 0.39c 4.28 ± 0.91ab 5.81 ± 0.81a 2.93 ± 0.44bc 1.30 ± 0.29c < 0.001 0.169 < 0.001
 T-SOD, U/mL 119.8 ± 1.4 121.0 ± 0.7 121.5 ± 0.5 122.0 ± 0.7 121.6 ± 0.5 121.4 ± 0.8 0.539 0.691 0.375
 GSH-PX, IU 1794 ± 102 1861 ± 68 1697 ± 76 1861 ± 53 1895 ± 94 1886 ± 71 0.484 0.391 0.812
Week 8
 MDA, nmol/mL 4.73 ± 0.63b 5.13 ± 0.53b 5.05 ± 0.54b 5.99 ± 0.44ab 4.39 ± 0.51b 6.91 ± 0.48a 0.040 0.153 0.240
 T-AOC, mmol/L 0.26 ± 0.02a 0.15 ± 0.03b 0.24 ± 0.05ab 0.22 ± 0.03ab 0.21 ± 0.02ab 0.17 ± 0.04ab 0.035 0.963 0.112
 NO, μmol/L 5.51 ± 1.07 5.27 ± 0.97 7.60 ± 0.98 10.44 ± 2.29 7.78 ± 1.92 5.25 ± 0.61 0.226 0.975 0.009
 TNOS, U/mL 38.8 ± 1.45 39.1 ± 0.78 38.5 ± 0.88 38.9 ± 1.48 33.3 ± 3.51 41.6 ± 1.24 0.075 0.974 0.110
 iNOS, U/mL 9.19 ± 0.95 9.60 ± 1.20 9.43 ± 0.41 9.06 ± 1.42 9.28 ± 1.87 9.87 ± 0.70 0.997 0.928 0.680
 CAT, U/mL 7.23 ± 1.62a 3.21 ± 0.48b 3.79 ± 0.72b 3.18 ± 0.42b 2.98 ± 0.50b 2.45 ± 0.48b 0.002 0.161 0.325
 T-SOD, U/mL 149.0 ± 4.8 137.7 ± 3.5 138.5 ± 4.2 145.4 ± 4.4 139.4 ± 4.8 143.9 ± 3.7 0.370 0.318 0.665
 GSH-PX, IU 2809 ± 149 3130 ± 212 2980 ± 149 2777 ± 118 2684 ± 96 2888 ± 82 0.306 0.124 0.198
  1. 1Data were presented as the mean ± SD (n = 15). Malondialdehyde (MDA) contents, Total antioxidant capacity (T-AOC), Nitric oxide (NO), Total nitric oxide synthase (tNOS) enzyme activity, Inducible nitric oxide synthase (iNOS) enzyme activity, Catalase (CAT) activity, Total superoxide dismutase (T-SOD) activity, Glutathione peroxidase (GSH-PX)
  2. 2The control hens were fed a diet with 16% protein level
  3. 3The experimental groups were provided a 14% protein diets (0.80% Arginine) supplemented with 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg
  4. 4Diet = effect of all the dietary treatments
  5. 5Linear and quadratic = effect of dietary arginine from 0.80% to 1.00% treatments
  6. a-c: Means with different superscripts within the same column differ significantly, P < 0.05