The optimal dietary arginine level of laying hens fed with low-protein diets

Table 2 Primers used for real-time quantitative PCR^a

Gene^b	Genebank accession no.	Orientation	Sequences (5′ to 3′)	Product size, bp
CAT1	NM_001145490.1	Forward	GCTCTATGGTGTTGGAGGG	192
CAT1	NM_001145490.1	Reverse	AATAAGCCACAAAGCAGATGAG	192
b^0,+AT	NM_001199133.1	Forward	TGTGTTGCTCTCTAACTGGCTG	154
b^0,+AT	NM_001199133.1	Reverse	CCTCCTTTCTGTTGTCCTGTTC	154
y⁺LAT1	XM_418326.5	Forward	TCCTGGTCATAGTTCCTCTCTACA	243
y⁺LAT1	XM_418326.5	Reverse	CAATGTCAAGGCAACCCTAC	243
rBAT	XM_004935370.2	Forward	TTGGCTTGGCAAAGGAGTC	146
rBAT	XM_004935370.2	Reverse	TCGGAATAGGCTGTGATGCT	146
B⁰AT	XM_419056	Forward	AATGGGACAACAAGGCTCAG	125
B⁰AT	XM_419056	Reverse	CAAGATGAAGCAGGGGGATA	125
EAAT3	XM_424930	Forward	ACCCTTTTGCCTTGGAAACT	122
EAAT3	XM_424930	Reverse	TTGAGATGTTTGCGTGAAG	122
PepT1	NM_204365	Forward	ACACGTTTGTTGCTCTGTGC	122
PepT1	NM_204365	Reverse	GACTGCCTGCCCAATTGTAT	122
GAPDH	NM_204305.1	Forward	ACATGGCATCCAAGGAGTGAG	144
GAPDH	NM_204305.1	Reverse	GGGGAGACAGAAGGGAACAGA	144

^aPCR = Polymerase chain reaction
^bCAT-1, Cationic amino acid transporter-1; b^0,+AT, b^0,+ amino acid transporter; y⁺LAT1, y⁺L amino acid transporter-1; rBAT, Related to b^0,+ amino acid transporter; B⁰AT, B⁰ neutral amino acid transporter; EAAT3, Acidic amino acids transporter; PepT1, Intestinal peptide transporter-1

ISSN: 2049-1891