Skip to main content

Table 1 α-Diversity metrics of ruminal multi-kingdom microbiota in heat-stressed (HS) cows and pair-fed thermoneutral (PFTN) control cows

From: Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows

Measurements Treatments (Trta) SEM P-values
PFTN HS Trt Period (P) Trt × P
Bacteria and archaea
 Chao1 estimates 259 249 14.57 0.706 0.437 0.061
 Observed ASVs 259 248 14.42 0.704 0.426 0.060
 Observed genera 62 55 2.78 0.210 0.181 0.342
 Observed species 66 59 3.02 0.222 0.194 0.370
 Evenness 0.87 0.87 0.01 0.927 0.045 0.935
 Faith’s phylogenetic diversity 21.57 19.92 0.98 0.365 0.274 0.204
 Shannon’s index 6.95 6.88 0.11 0.724 0.124 0.406
 Simpson’s index 0.98 0.98 0 0.992 0.105 0.770
Fungi
 Chao1 estimates 81 83 5.31 0.837 0.155 0.327
 Observed ASVs 81 83 5.32 0.888 0.136 0.358
 Observed genera 44 43 3.82 0.897 0.105 0.584
 Observed species 50 50 4.66 0.979 0.085 0.592
 Evenness 0.69 0.73 0.02 0.222 0.414 0.164
 Faith’s phylogenetic diversity 20.05 20.10 1.48 0.987 0.084 0.418
 Shannon’s index 4.31 4.67 0.14 0.184 0.123 0.245
 Simpson’s index 0.89 0.92 0.01 0.100 0.900 0.145
Protozoa
 Chao1 estimates 19 19 0.874 0.819 0.631 0.806
 Observed ASVs 19 19 0.861 0.793 0.602 0.746
 Observed genera 5.5b 6.13a 0.164 0.004 0.473 0.089
 Observed species 10 10 0.272 0.633 0.074 0.347
 Evenness 0.66 0.53 0.044 0.057 0.001 0.574
 Faith’s phylogenetic diversity 1.56 1.42 0.172 0.732 0.976 0.630
 Shannon’s index 2.76 2.27 0.187 0.075 0.001 0.483
 Simpson’s index 0.76 0.65 0.047 0.134 0.009 0.377
  1. * Thermal treatment (heat stress vs. pair-fed thermoneutrality)
  2. Good’s coverage of all samples was ≥99.9%
  3. Mean values with different superscript in a row differ (P < 0.05)