Fig. 1

Identification of DAZL::GFP cells and single-cell transcriptome profiling in DAZL::GFP chickens at E12, E16, and hatch. A Illustration of experimental workflow. B Immunohistochemistry of left testes of the DAZL::GFP chickens at E12, E16, and hatch. Scale bars, 20 μm (upper row) and 40 μm (bottom row). C Hematoxylin and eosin staining of left testes of DAZL::GFP chickens at E12, E16, and hatch. Scale bars, 20 μm (upper row) and 40 μm (bottom row). D Uniform manifold approximation and projection (UMAP) plot of DAZL::GFP cells from testes of DAZL::GFP chickens at E12, E16, and hatch. E UMAP plots illustrating expression of the germ cell markers (DAZL, DDX4, and PIWIL1), pluripotency markers (NANOG, Pou5f3, and SOX2), and mitotic-germ cell markers (CFC1, TFAP2C, and CXCR4) at E12, E16, and hatch. The dotted lines on UMAP plots indicate the approximate division between the three samples, the right part is E12, the middle part is E16, and the lower-left part is hatch