Fig. 4

FFA inhibited translocation of NFE2L2 into the nucleus in MAC-T cells. MAC-T cells were treated with 0, 0.6, 1.2 or 2.4 mmol/L FFA for 24 h. a Western blot analysis of nuclear NFE2L2 in MAC-T cells. b Protein abundance of nuclear NFFE2L2. c MAC-T cells were treated with 1.2 mmol/L FFA for 24 h. Immunofluorescence for NFE2L2 (red) was performed, and the nuclear dye DAPI (blue) was used, scale bar = 25 μm. Comparisons among groups were calculated using a one-way ANOVA with subsequent Bonferroni correction. The data presented are the mean ± SEM. Different superscript lowercase letters in bar charts represent significant difference (P < 0.05)