Associations between testicular development and fetal size in the pig

Stenhouse, Claire; Cortes-Araya, Yennifer; Donadeu, F. Xavier; Ashworth, Cheryl J.

doi:10.1186/s40104-022-00678-3

Journal of Animal Science and Biotechnology

Table 1 Primer sequence details for qPCRs

From: Associations between testicular development and fetal size in the pig

Gene symbol	Gene name	Accession number	Primer sequence (5′➔ 3′)		Amplicon size, bp	Reference
BAX	BCL2 associated X	XM_003127290.5	Fwd	CCGAAATGTTTGCTGACG	154	[25]
BAX	BCL2 associated X	XM_003127290.5	Rev	AGCCGATCTCGAAGGAAGT	154	[25]
BCL2	B-cell lymphoma 2	XM_021099593.1	Fwd	GATAACGGAGGCTGGGATGC	147	[26]
BCL2	B-cell lymphoma 2	XM_021099593.1	Rev	CTTATGGCCCAGATAGGCACC	147	[26]
CD31	Platelet and Endothelial Cell Adhesion Molecule 1	NM_213907.1	Fwd	CCGAGGTCTGGGAACAAAGG	98	[26]
CD31	Platelet and Endothelial Cell Adhesion Molecule 1	NM_213907.1	Rev	AGCCTTCCGTTCTAGAATATCTGTT	98	[26]
DMRT1	Doublesex and mab-3 related transcription factor 1	NM_214111.1	Fwd	AGTGTATTGTCGCCACCCAG	92	[26]
DMRT1	Doublesex and mab-3 related transcription factor 1	NM_214111.1	Rev	AGCACTCCCTTTGTGCTCTC	92	[26]
GATA4	GATA-binding protein 4	NM_214293.1	Fwd	CGACACCCTAATCTCGATATGTT	156	[26]
GATA4	GATA-binding protein 4	NM_214293.1	Rev	CCGGCTGATGCCATTCATCT	156	[26]
HIF1A	Hypoxia Inducible Factor 1 Alpha Subunit	NM_001123124	Fwd	CCATGCCCCAGATTCAAGAT	64	[27]
HIF1A	Hypoxia Inducible Factor 1 Alpha Subunit	NM_001123124	Rev	GGTGAACTCTGTCTAGTGCTTCCA	64	[27]
KI67	Ki67	NM_001101827.1	Fwd	AGTCTGTAAGGAAAGCCACCC	119	[26]
KI67	Ki67	NM_001101827.1	Rev	ACAAAGCCCAAGCAGACAGG	119	[26]
P53	Tumour protein p53	NM_213824.3	Fwd	GCCACTGGATGGCGAGTATT	84	[26]
P53	Tumour protein p53	NM_213824.3	Rev	TCCAAGGCGTCATTCAGCTC	84	[26]
SPP1	Secreted Phosphoprotein 1	X16575	Fwd	TTGGACAGCCAAGAGAAGGACAGT	121	[28]
SPP1	Secreted Phosphoprotein 1	X16575	Rev	GCTCATTGCTCCCATCATAGGTCTTG	121	[28]
TBP1	TATA box binding protein	DQ845178	Fwd	AACAGTTCAGTAGTTATGAGCCAGA	153	[29]
TBP1	TATA box binding protein	DQ845178	Rev	AGATGTTCTCAAACGCTTCG	153	[29]
VEGFA	Vascular Endothelial Growth Factor A	NM_214084	Fwd	GCCCACTGAGGAGTTCAACATC	59	[27]
VEGFA	Vascular Endothelial Growth Factor A	NM_214084	Rev	GGCCTTGGTGAGGTTTGATC	59	[27]
WYHAZ	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide	DQ845179	Fwd	TGATGATAAGAAAGGGATTGTGG	203	[29]
WYHAZ		DQ845179	Rev	GTTCAGCAATGGCTTCATCA	203	[29]

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ISSN: 2049-1891

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