Table 1 The primers sequences for RT-PCR and SELECT
Target genes | Primer sequences (5′ to 3′) | |
|---|---|---|
CLOCK | F: GATCACAGGGCACCTCCAATA | R: CTAGTTCTCGCCGCCTTTCT |
B1AML1 | F: GTAGACCAGAGGGCGACAG | R: ATGAAACTGAACCAGCGACTC |
CRY1 | F: GATGTGGCTATCCTGTAGTTCCT | R: GCTGCTGGTAGATTTGTTTCAT |
CRY2 | F: GCACGGCTGGATAAACACT | R: AAATAAGCGGCAGGACAAA |
PER2 | F: ATGAAACGAGCCATCCCG | R: CAGTTGTCGTGATTTTGCCTA |
PER3 | F: CAGTGCCTTTGTTGGGTTAC | R: GATGGATTCACAAAACTGGAC |
CRH | F: CTCCCTGGGCCTGGCTTT | R: CCTCACTTCCCGATGATT |
CRHR1 | F: CACAGCCTTCATCCTACGCA | R: CGGAGCTTGTCGGTGGAATA |
CRHR2 | F: TCTTTCCTGGGCTTTCACGG | R: ATTGAAGAACTCCGGGCAGG |
NPY | F: ACTCGGCTCTGAGGCACT | R: GGTCTTCAAACCGGGATC |
FTO | F: TCACCAAGGCGACCTCTACT | R: GCTGAACCGAGGTGAAAAGC |
METTL3 | F: ATCCTGGAGCTGCTCAACAC | R: AGATTCGTCCGTGTGCTTGT |
METTL14 | F: ATTCGACCAGGATGGCTGAC | R: GACTTGGGTGGTGGTGACTT |
YTHDF1 | F: AACAACCAGCTCCGACACAT | R: GATTCTGACGTTCCTTCCGC |
YTHDF2 | F: AAGGCCAAGGCAACAAAGTG | R: ATATGCATTGTTCGGCCGGG |
YTHDF3 | F: CGTAATAGGGGTGTGGGCTTC | R: CACTTCCACACCAGAAGGTGA |
PPIA | F: TTACGGGGAGAAGTTTGCCG | R: TGGTGATCTGCTTGCTCGTC |
SELECT | ||
 CRH N site | F: tagccagtaccgtagtgcgtgGGCGCGCAGCGCGGCCGCTG R: CCCGGTGCTGAAACGCGGCCcagaggctgagtcgctgcat | |
 CRH X1 site | F: tagccagtaccgtagtgcgtgTTCCCGATGATTTCCATCAG R: TTCCTGTTGCTGTGGGCTTGcagaggctgagtcgctgcat | |
 CRH X2 site | F: tagccagtaccgtagtgcgtgCTCTGGTGCTGACCGCGGGG R: CCCTTTGGCACGGCGCGGGGcagaggctgagtcgctgcat | |