Fig. 6
Effect of chronic CORT exposure on the site-specific m6A levels in the 3’UTR of CRH mRNA in chicken hypothalamus. Validation of m6A modification in CRH 3’UTR using single-base elongation and ligation-based qPCR amplification method (SELECT) when treatment with CORT in chicken hypothalamus. (A) Schematic graph of N, X1 and X2 site in CRH gene; (B) Amplification curve and qPCR CT value in CRH N site at ZT18; (C) Amplification curve and qPCR CT value in CRH X1 site at ZT18; (D) Amplification curve and qPCR CT value in CRH X2 site at ZT18; (E) Amplification curve and qPCR CT value in CRH N site at ZT22; (F) Amplification curve and qPCR CT value in CRH X1 site at ZT22; (G) Amplification curve and qPCR CT value in CRH X2 site at ZT22. Values are mean ± SEM, n = 6 chickens per time point. *P < 0.05, ** P < 0.01, compared with control