Fig. 3

Functional enrichment analysis of differentially expressed genes (DEGs) identified in the comparisons of embryo groups: vitrification versus in vitro culture (VIT vs. IVC), in vitro culture versus control (IVC vs. CO) and vitrification versus in vivo control (VIT vs. CO), and using Metascape tool. A Heatmaps representing visualization of top 100 clusters of enriched terms across DEG lists with selected DEG (cut–off FDR < 0.001 and log2FC ≥ 2. In all plots, bar graph of enriched terms across DEG colored by P-values representing enriched clusters for score of ≥2. B Functional membership analysis for differentially expressed genes (DEG) with cut off FDR ≤ 0.001 identified in the comparisons of embryo groups vitrification versus in vivo control (VIT vs. CO); in vitro culture versus control (IVC vs. CO); and vitrification versus in vitro culture (VIT vs. IVC) using Metascape tool. Enrichment of DEG matching membership terms: “embryo”, “ oxidative damage”, “cell arrest” and “stress response”. The outer pie shows the number and the percentage of DEG in the background that are associated with the membership (in black); the inner pies show the number and the percentage of DEG in the individual input gene list that are associated with the membership. The P-values on the top of the pie charts indicates whether the membership is statistically significantly enriched in the list