Table 2 Primer sequences of housekeeping and target genes concerned with intestinal barrier function
Item1 | Sequences (5′ to 3′)2 | Length, bp | Tm, °C |
|---|---|---|---|
Occludin | F: GTGGGACAAGGAACGTATT R: TCTCTCCGCATAGTCCGAA | 115 | 60 |
Claudin-1 | F: ATACAGGAGGGAAGCCAT R: ATATATTTAAGGACCGCCCTCT | 89 | 60 |
ZO-1 | F: GCTCAGCCCTATCCATCT R: GGACGGGACCTGCTCATAA | 90 | 60 |
Mucin-1 | F: GTGCCGCTGCCCACAACCTG R: AGCCGGGTACCCCAGACCCA | 141 | 60 |
Mucin-2 | F: CAGACCTACTCAGAGTTCCT R: CTCGGGCTTGTTGATCTT | 84 | 60 |
GAPDH | F: CAGCAATGCCTCCTGTACCA | 72 | 60 |
R: ACGATGCCGAAGTTGTCATG |