Dietary branched-chain amino acids modulate the dynamics of calcium absorption and reabsorption in protein-restricted pigs

Table 2 The host, dilution, and supplier of primary and secondary antibodies for immunoblotting

Antibodies	Host	Dilution	Vendor
Anti-NHE3¹	Rabbit	1:375	ThermoFisher Scientific, Rockford, IL, USA #27190–1-AP
Anti-VDR¹	Rat	1:150	ThermoFisher Scientific, Rockford, IL, USA #MA5–14614
Anti-CLDN-1	Rabbit	1:300	ThermoFisher Scientific, Rockford, IL, USA #71–7800
Anti-CYP27B1¹	Rabbit	1:2000	ThermoFisher Scientific, Rockford, IL, USA #PA5–79128
Anti-GAPDH	Mouse	1:7500	Abcam, Cambridge, MA, USA #ab105428
Anti-Rabbit IgG H&L (HRP)	Goat	1:7500	Abcam, Cambridge, MA, USA #ab205718
Anti-Rat IgG (HRP)	Rabbit	1:3000	Abcam, Cambridge, MA, USA #ab6734

¹ NHE3, Na⁺/H⁺ exchanger 3; VDR, vitamin D receptor; CLDN-1, claudin-1; CYP27B1, cytochrome P450 family 27 subfamily B member 1

ISSN: 2049-1891