Fig. 5
The effects of RyR1-knockout on cellular Ca2+ dynamics and differentiation of C2C12 myoblasts. (A-C) The concentration of Ca2+ in cytoplasm (labeled by Fluo-3, n = 3) and endoplasmic reticulum (labeled by Mag-fluo-AM, n = 5) of RyR1-KO cells. (D) The effect of RyR1-KO on the mRNA expression of Ca2+ channels (n = 3). (E-G) Proliferation and viability of RyR1-KO cells measured by EdU staining or CCK-8 test (n = 10). (H) Relative mRNA expression of Myf5, MyoD1, and MyoG in RyR1-KO cells (n = 3). (I-J) The expression of proteins related to MAPK signaling in RyR1-KO cells (n = 3). RyR1−/− and RyR1+/− represent homozygote and heterozygote of RyR1-knockout respectively. The data are presented as the mean ± SEM. *Represents significant difference between the two groups (P < 0.05)