Fig. 7

The miR-499-3p/NAMPT/AMPK axis is required for the function of circPTPN4. (A and B) Protein expression levels of AMPK signaling with circPTPN4 overexpression (A) or interference (B). The numbers shown below the bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to β-Tubulin. Data are expressed as a fold-change relative to the control. (C to G) Relative mRNA expression of NAMPT (C), relative mRNA levels of several cell cycle genes (D), relative mRNA expression levels of myoblast differentiation marker genes (E), relative mRNA expression levels of glycogenolytic and glycolytic genes (F), and relative mRNA expression levels of several fast−/slow-twitch myofiber genes (G) induced by the listed nucleic acids in CPMs. Results are shown as mean ± SEM. In panels (C to G), the statistical significance of differences between means was assessed using ANOVA followed by Dunnett’s test. (*P < 0.05; **P < 0.01)