Fig. 2

Alleviation of necrotic enteritis by butyrate and forskolin (FSK). Day-10 male Cobb broilers were supplemented with or without microencapsulated sodium butyrate (1 g/kg diet), FSK-containing Coleus forskohlii (CF) extract (10 mg/kg) individually or in combination (butyrate plus 5 or 10 mg/kg CF extract) with 3 cages per treatment and 3 animals per cage (n = 9), followed by daily challenges with Clostridium perfringens (CP) from d 14 to 17. Chickens were individually weighted on d 13 (A) and d 18 (B), and gross lesions of necrotic enteritis in the duodenum (C) and jejunum (D) were scored on d 18. The CP titers in the digesta of the jejunum (E) and cecum (F) were quantified using qPCR. Results were presented as means ± SEM (n = 9). Statistical significance (P < 0.05), denoted by uncommon superscripts, was determined using one-way ANOVA and post hoc Tukey’s test