Fig. 4

Oleic acid increases triglyceride (TG) levels and reduces cholesteryl ester (CE) levels in mice ovarian tissues. The female mice were fed with a 20%-oleic acid or negative control diet (NC) for 8 weeks, and tissues were dissected after the animals were sacrificed. A Oil Red O staining of ovarian tissues (n = 3), scale bars = 100 μm and 200 μm; **, antral follicle; §, corpora lutea. B Detection of the TG and CE levels in the ovaries (n = 5); C Representative images of H&E-stained sections of peri-ovarian adipose, gonadal adipose and liver tissues, scale bar = 200 μm; D Adipocyte area statistics of peri-ovarian adipose tissue (n = 5); E Adipocyte area statistics of gonadal adipose tissue (n = 5). F The sera of female mice were collected to determine the statistics of TG, CE, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) levels (n = 5). Note: Data are represented as the means ± SEMs. Significance was determined using Student’s t-test, *P < 0.05, **P < 0.01