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Table 4 Total and differential white blood cells, and serum cytokine and acute-phase proteins in ETEC-infected weaned pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics

From: Supplementation of oligosaccharide-based polymer enhanced growth and disease resistance of weaned pigs by modulating intestinal integrity and systemic immunity

 

Diet

 

P-value

Item1

Control

LOW2

HIGH3

CAR4

SEM

Diet

Linear5

d 0 before infection

 WBC, 103/μL

15.28

16.91

15.70

16.85

1.00

0.58

0.77

 Neu, 103/μL

7.93

9.03

8.43

7.56

0.67

0.46

0.60

 Lym, 103/μL

6.39b

6.72b

6.08b

8.26a

0.48

< 0.05

0.65

 Mono, 103/μL

0.66

0.83

0.79

0.75

0.11

0.73

0.41

 Eos, 103/μL

0.24

0.27

0.35

0.20

0.10

0.64

0.33

 Baso, 103/μL

0.057

0.072

0.051

0.075

0.018

0.074

0.81

Serum

 TNF-α, pg/mL

63.56

59.67

61.51

72.80

1.62

0.98

0.99

 C-reactive protein, μg/mL

7.37

6.86

8.57

8.08

1.39

0.86

0.46

 Haptoglobin, g/mL

0.984

0.730

1.211

1.072

0.135

0.065

0.031

d 2 PI

 WBC, 103/μL

21.16a

18.04ab

17.78b

18.53ab

1.01

< 0.05

< 0.05

 Neu, 103/μL

12.25a

9.83b

9.79b

8.34b

0.96

< 0.05

< 0.05

 Lym, 103/μL

7.27ab

6.97b

6.79b

8.54a

0.41

< 0.05

0.41

 Mono, 103/μL

1.16

1.05

0.85

1.33

0.16

0.29

0.21

 Eos, 103/μL

0.40

0.25

0.19

0.23

0.10

0.09

< 0.05

 Baso, 103/μL

0.106a

0.036b

0.039b

0.029b

0.017

< 0.05

< 0.05

Serum

 TNF-α, pg/mL

107.92

67.06

66.28

66.28

32.89

0.63

0.28

 C-reactive protein, μg/mL

20.42a

22.74a

22.88a

12.38b

2.693

0.018

0.65

 Haptoglobin, g/mL

1.306ab

1.142b

1.561a

1.066b

0.127

< 0.05

0.11

d 5 PI

 WBC, 103/μL

21.00

18.56

18.67

18.19

1.09

0.14

0.15

 Neu, 103/μL

10.93a

9.17b

9.54ab

7.89b

0.54

< 0.05

< 0.05

 Lym, 103/μL

9.03

8.22

8.02

8.35

0.65

0.69

0.25

 Mono, 103/μL

0.86

0.86

0.79

1.21

0.15

0.29

0.74

 Eos, 103/μL

0.13b

0.26ab

0.25ab

0.55a

0.14

< 0.05

0.41

 Baso, 103/μL

0.059

0.043

0.078

0.057

0.016

0.49

0.41

Serum

 TNF-α, pg/mL

90.64

68.36

54.39

32.99

32.88

0.25

0.16

 C-reactive protein, μg/mL

21.61a

17.46ab

19.84ab

15.96b

1.72

0.082

0.93

 Haptoglobin, g/mL

1.655a

1.084b

1.348ab

1.154b

0.128

0.081

0.93

d 11 PI

 WBC, 103/μL

16.15

17.40

17.32

14.92

1.58

0.64

0.57

 Neu, 103/μL

8.68

10.71

9.67

8.12

1.22

0.16

0.40

 Lym, 103/μL

6.33

6.69

7.21

6.39

0.63

0.76

0.35

 Mono, 103/μL

0.66

1.12

0.99

1.39

0.19

0.10

0.21

 Eos, 103/μL

0.18

0.31

0.24

0.21

0.13

0.74

0.57

 Baso, 103/μL

0.058

0.083

0.048

0.042

0.025

0.51

0.70

Serum

 TNF-α, pg/mL

82.58

84.62

72.71

59.39

28.03

0.96

0.79

 C-reactive protein, μg/mL

21.95a

17.71ab

18.29ab

12.61b

2.89

0.091

0.65

 Haptoglobin, g/mL

0.819

0.599

0.538

0.412

0.178

0.24

0.31

  1. a,bWithin a row, means without a common superscript differ (P < 0.05)
  2. 1WBC white blood cell, Neu neutrophil, Lym lymphocyte, Mono monocyte, Eos eosinophil, Baso basophil, PI post-inoculation. Each least squares mean represents 12 observations, except d 11 PI that has 6 observations
  3. 2LOW Low dose blood group A6 type 1-based polymer (Coligo)
  4. 3HIGH High dose blood group A6 type 1-based polymer (Coligo)
  5. 4CAR carbadox
  6. 5Linear effects of adding Coligo to the control diet