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Table 8 The effect of CAL on ruminal fermentation in cannulated lambs (Exp. 2)

From: Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs

Parameters 0 h 3 h 6 h Group SEM P-value
CON CAL SEM CON CAL SEM CON CAL SEM CON CAL D H D × H
pH 6.87 7.05 0.09 5.95 6.39 0.06 6.34 6.58 0.04 6.39 6.67 0.05 < 0.01 < 0.01 0.20
NH3, mmol/L 8.75 10.4 0.33 9.34 11.2 0.45 5.85 8.93 0.57 7.98 10.2 0.30 < 0.01 < 0.01 0.27
Total VFA, mmol/L 66.8 67.9 2.77 103.6 101.3 3.83 83.4 86.9 1.50 84.6 85.4 2.36 0.79 < 0.01 0.73
VFA, molar percent
 Acetate (A) 71.6 69.8 0.39 66.2 66.4 0.41 67.4 67.7 0.44 68.4 67.9 0.32 0.29 < 0.01 0.08
 Propionate (P) 15.6 d 17.5c 0.34 16.3 d 20.1a 0.49 15.7d 18.9b 0.39 15.9 18.8 0.25 < 0.01 < 0.01 0.04
 Isobutyrate 0.42 0.61 0.07 0.52 0.93 0.10 0.46 0.76 0.07 0.47 0.77 0.05 < 0.01 0.15 0.61
 Butyrate 10.3b 10.5b 0.11 14.9a 11.1b 0.50 14.4a 11.2b 0.51 13.2 11.0 0.28 < 0.01 < 0.01 < 0.01
 Isovalerate 1.00 0.75 0.05 0.53 0.41 0.02 0.62 0.44 0.03 0.72 0.53 0.03 < 0.01 < 0.01 0.31
 Valerate 1.11c 0.92d 0.04 1.59a 1.07cd 0.07 1.36b 1.03cd 0.07 1.36 1.01 0.04 < 0.01 < 0.01 0.01
A/P ratio 4.61 4.04 0.11 4.07 3.33 0.10 4.29 3.61 0.09 4.33 3.66 0.06 < 0.01 < 0.01 0.71
  1. CON control diet, CAL Coleus amboinicus Lour. diet, SEM standard error of means. D diet, H hour, VFA volatile fatty acids
  2. Means in the same row indicate significant differences at P < 0.05 and tended to significant at P < 0.10