Fatty acid, % of total FA | Rumen fluid | LT muscle | ||||||
---|---|---|---|---|---|---|---|---|
CON | CAL | SEM | P value | CON | CAL | SEM | P-value | |
Saturated FA | ||||||||
 C8:0 | 0.03 | 0.01 | 0.01 | 0.39 | 1.44 | 0.60 | 0.39 | 0.31 |
 C10:0 | 0.08 | 0.49 | 0.14 | 0.16 | 1.85 | 1.49 | 0.41 | 0.68 |
 C12:0 | 0.64 | 0.98 | 0.13 | 0.19 | 1.62 | 1.23 | 0.26 | 0.48 |
 C13:0 | 1.87 | 1.94 | 0.17 | 0.86 | 1.09 | 0.82 | 0.26 | 0.63 |
 C14:0 | 1.11 | 1.29 | 0.14 | 0.56 | 1.77 | 1.62 | 0.26 | 0.79 |
 C15:0 | 1.46 | 1.44 | 0.13 | 0.92 | 1.13 | 1.11 | 0.22 | 0.96 |
 C16:0 | 16.5 | 18.7 | 0.49 | 0.01 | 14.1 | 12.5 | 0.50 | 0.11 |
 C17:0 | 0.73 | 0.62 | 0.07 | 0.48 | 0.87 | 1.07 | 0.13 | 0.48 |
 C18:0 | 47.3 | 40.9 | 1.59 | 0.03 | 15.4 | 12.1 | 0.73 | 0.01 |
 C20:0 | 0.06 | 0.06 | 0.02 | 0.86 | 0.21 | 0.20 | 0.04 | 0.88 |
 C21:0 | 0.05 | 0.11 | 0.02 | 0.17 | 0.26 | 0.38 | 0.07 | 0.41 |
 C22:0 | 0.11 | 0.08 | 0.02 | 0.37 | 0.52 | 0.65 | 0.08 | 0.44 |
 C23:0 | 0.09 | 0.21 | 0.03 | 0.02 | 0.34 | 0.71 | 0.11 | 0.10 |
 C24:0 | 0.56 | 0.55 | 0.03 | 0.87 | 0.18 | 0.50 | 0.09 | 0.09 |
Monounsaturated FA | ||||||||
 C14:1 | 0.24 | 0.28 | 0.06 | 0.76 | 0.69 | 0.96 | 0.17 | 0.45 |
 C15:1 | 1.11 | 0.87 | 0.12 | 0.32 | 1.57 | 1.49 | 0.20 | 0.84 |
 C16:1 | 0.31 | 0.32 | 0.05 | 0.96 | 1.03 | 0.99 | 0.14 | 0.89 |
 C17:1 | 0.17 | 0.17 | 0.05 | 0.99 | 1.24 | 1.71 | 0.18 | 0.20 |
 C18:1 trans-10 | 0.62 | 0.48 | 0.04 | 0.05 | 0.30 | 0.21 | 0.03 | 0.12 |
 C18:1 trans-11 (VA) | 5.25 | 4.41 | 0.30 | 0.19 | 1.07 | 0.75 | 0.10 | 0.11 |
 C18:1 cis-9 | 6.98 | 7.53 | 0.37 | 0.49 | 22.1 | 22.4 | 1.11 | 0.92 |
 C18:1 cis-11 | 1.65 | 1.68 | 0.06 | 0.83 | 2.30 | 2.17 | 0.12 | 0.62 |
 C18:1 cis-12 | 0.57 | 0.68 | 0.06 | 0.46 | 0.77 | 0.62 | 0.08 | 0.37 |
 C18:1 cis-13 | 0.12 | 0.19 | 0.03 | 0.26 | 0.23 | 0.21 | 0.05 | 0.86 |
 C18:1 cis-14 | 1.00 | 0.81 | 0.10 | 0.36 | 0.63 | 0.50 | 0.11 | 0.57 |
 C20:1 trans- | 0.84 | 0.90 | 0.03 | 0.36 | 0.52 | 0.64 | 0.11 | 0.64 |
 C22:1 n-9 | 0.16 | 0.09 | 0.03 | 0.22 | 0.38 | 0.60 | 0.09 | 0.23 |
 C24:1 | 0.14 | 0.71 | 0.11 | < 0.01 | 1.44 | 1.92 | 0.14 | 0.10 |
Polyunsaturated FA | ||||||||
 C18:2 cis-12 trans-10 (CLA-t10) | 0.17 | 0.66 | 0.15 | 0.18 | 0.51 | 0.56 | 0.10 | 0.82 |
 C18:2 cis-9 trans-11 (RA) | 0.49 | 0.37 | 0.05 | 0.21 | 0.44 | 0.69 | 0.11 | 0.27 |
 C18:2 cis-9 cis-12 (LA) | 6.42 | 8.07 | 0.40 | 0.02 | 15.1 | 17.6 | 0.93 | 0.19 |
 C18:3 cis-6 cis-9 cis-12 | 0.15 | 0.27 | 0.04 | 0.11 | 0.49 | 0.57 | 0.09 | 0.67 |
 C18:3 cis-9 cis-12 cis-15 (LNA) | 1.47 | 2.28 | 0.16 | < 0.01 | 1.05 | 1.73 | 0.19 | 0.05 |
 C20:2 | 0.43 | 0.37 | 0.06 | 0.63 | 0.35 | 0.44 | 0.10 | 0.68 |
 C20:3 n-6 | 0.63 | 0.65 | 0.07 | 0.89 | 4.04 | 5.11 | 0.38 | 0.17 |
 C20:4 n-6 | nd. | nd. | nd. | nd. | 0.51 | 0.42 | 0.05 | 0.44 |
 C20:5 n-3 | nd. | nd. | nd. | nd. | 0.74 | 0.64 | 0.14 | 0.73 |
 C22:2 | 0.08 | 0.16 | 0.04 | 0.28 | 0.62 | 0.49 | 0.15 | 0.72 |
 C22:5 n-3 | nd. | nd. | nd. | nd. | 0.64 | 0.90 | 0.09 | 0.17 |
 C22:6 n-3 | nd. | nd. | nd. | nd. | 0.45 | 0.79 | 0.15 | 0.27 |
∑ SFA | 70.5 | 67.3 | 0.96 | 0.10 | 40.8 | 34.9 | 1.24 | 0.01 |
∑ UFA | 29.5 | 32.7 | 0.96 | 0.10 | 59.2 | 65.1 | 1.24 | 0.01 |
∑ MUFA | 18.7 | 20.1 | 0.60 | 0.66 | 34.3 | 35.1 | 0.95 | 0.70 |
∑ PUFA | 10.8 | 12.5 | 0.51 | < 0.01 | 24.9 | 30.0 | 1.01 | 0.07 |
∑ n-6 | 8.90 | 9.72 | 0.42 | 0.02 | 21.6 | 25.2 | 1.23 | 0.17 |
∑ n-3 | 1.47 | 2.28 | 0.16 | < 0.01 | 2.88 | 4.06 | 0.31 | 0.07 |
n-6/n-3 | 6.05 | 4.37 | 0.39 | 0.02 | 7.86 | 6.74 | 0.73 | 0.47 |
PUFA/SFA | 0.15 | 0.19 | 0.01 | 0.07 | 0.62 | 0.86 | 0.05 | 0.01 |
LNA/LA | 0.21 | 0.30 | 0.02 | 0.02 | 0.07 | 0.10 | 0.01 | 0.31 |
∑ C18:1 | 15.7 | 16.8 | 0.64 | 0.45 | 27.4 | 26.8 | 1.22 | 0.81 |
∑ C18:1 trans- | 5.48 | 5.08 | 0.28 | 0.52 | 1.37 | 0.96 | 0.11 | 0.06 |
∑ C18:1 cis- | 10.2 | 11.7 | 0.58 | 0.23 | 26.1 | 25.8 | 1.19 | 0.94 |
∑ CLA | 0.64 | 0.99 | 0.19 | 0.36 | 0.95 | 1.61 | 0.18 | 0.04 |
∑ MCFA | 23.3 | 26.3 | 0.94 | 0.12 | 26.3 | 22.8 | 1.25 | 0.17 |
∑ LCFA | 76.7 | 73.7 | 0.94 | 0.12 | 73.7 | 77.2 | 1.25 | 0.17 |
BH intermediates | 7.81 | 7.76 | 0.46 | 0.95 | nd. | nd. | nd. | nd. |
LA BH, % | 74.2 | 67.6 | 1.58 | 0.03 | nd. | nd. | nd. | nd. |
LNA BH, % | 94.4 | 91.5 | 0.61 | < 0.01 | nd. | nd. | nd. | nd. |
RA/VA | 0.08 | 0.11 | 0.02 | 0.50 | nd. | nd. | nd. | nd. |
Desaturation index (DI) ∆9 | nd. | nd. | nd. | nd. | 0.41 | 0.47 | 0.01 | 0.01 |
D∆9. C14:1/C14:0 | nd. | nd. | nd. | nd. | 0.29 | 0.37 | 0.06 | 0.50 |
D∆9. 16:1/16:0 | nd. | nd. | nd. | nd. | 0.07 | 0.07 | 0.01 | 0.85 |
D∆9. 18:1/18:0 | nd. | nd. | nd. | nd. | 0.59 | 0.65 | 0.01 | 0.01 |
D∆9. RA/VA | nd. | nd. | nd. | nd. | 0.27 | 0.48 | 0.06 | 0.04 |
D∆9. MUFA/SFA | nd. | nd. | nd. | nd. | 0.46 | 0.50 | 0.01 | 0.08 |
D∆5. n-6. 20:4n-6/20:3n-6 | nd. | nd. | nd. | nd. | 0.12 | 0.08 | 0.01 | 0.11 |
D∆5. D6. n-6. 20:4n-6/18:3n-6 | nd. | nd. | nd. | nd. | 0.53 | 0.43 | 0.06 | 0.43 |
D∆4. n-3. 22:6n-3/22:5n-3 | nd. | nd. | nd. | nd. | 0.33 | 0.41 | 0.06 | 0.53 |
Elongase index | nd. | nd. | nd. | nd. | 0.71 | 0.72 | 0.01 | 0.92 |
Thrombogenic index | nd. | nd. | nd. | nd. | 0.85 | 0.62 | 0.05 | < 0.01 |
Atherogenicity index | nd. | nd. | nd. | nd. | 0.63 | 0.48 | 0.03 | 0.03 |