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Table 12 Fatty acid profile in rumen fluid and longissimus thoracis muscle of lambs fed CAL (Exp. 3)

From: Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs

Fatty acid, % of total FA Rumen fluid LT muscle
CON CAL SEM P value CON CAL SEM P-value
Saturated FA
 C8:0 0.03 0.01 0.01 0.39 1.44 0.60 0.39 0.31
 C10:0 0.08 0.49 0.14 0.16 1.85 1.49 0.41 0.68
 C12:0 0.64 0.98 0.13 0.19 1.62 1.23 0.26 0.48
 C13:0 1.87 1.94 0.17 0.86 1.09 0.82 0.26 0.63
 C14:0 1.11 1.29 0.14 0.56 1.77 1.62 0.26 0.79
 C15:0 1.46 1.44 0.13 0.92 1.13 1.11 0.22 0.96
 C16:0 16.5 18.7 0.49 0.01 14.1 12.5 0.50 0.11
 C17:0 0.73 0.62 0.07 0.48 0.87 1.07 0.13 0.48
 C18:0 47.3 40.9 1.59 0.03 15.4 12.1 0.73 0.01
 C20:0 0.06 0.06 0.02 0.86 0.21 0.20 0.04 0.88
 C21:0 0.05 0.11 0.02 0.17 0.26 0.38 0.07 0.41
 C22:0 0.11 0.08 0.02 0.37 0.52 0.65 0.08 0.44
 C23:0 0.09 0.21 0.03 0.02 0.34 0.71 0.11 0.10
 C24:0 0.56 0.55 0.03 0.87 0.18 0.50 0.09 0.09
Monounsaturated FA
 C14:1 0.24 0.28 0.06 0.76 0.69 0.96 0.17 0.45
 C15:1 1.11 0.87 0.12 0.32 1.57 1.49 0.20 0.84
 C16:1 0.31 0.32 0.05 0.96 1.03 0.99 0.14 0.89
 C17:1 0.17 0.17 0.05 0.99 1.24 1.71 0.18 0.20
 C18:1 trans-10 0.62 0.48 0.04 0.05 0.30 0.21 0.03 0.12
 C18:1 trans-11 (VA) 5.25 4.41 0.30 0.19 1.07 0.75 0.10 0.11
 C18:1 cis-9 6.98 7.53 0.37 0.49 22.1 22.4 1.11 0.92
 C18:1 cis-11 1.65 1.68 0.06 0.83 2.30 2.17 0.12 0.62
 C18:1 cis-12 0.57 0.68 0.06 0.46 0.77 0.62 0.08 0.37
 C18:1 cis-13 0.12 0.19 0.03 0.26 0.23 0.21 0.05 0.86
 C18:1 cis-14 1.00 0.81 0.10 0.36 0.63 0.50 0.11 0.57
 C20:1 trans- 0.84 0.90 0.03 0.36 0.52 0.64 0.11 0.64
 C22:1 n-9 0.16 0.09 0.03 0.22 0.38 0.60 0.09 0.23
 C24:1 0.14 0.71 0.11 < 0.01 1.44 1.92 0.14 0.10
Polyunsaturated FA
 C18:2 cis-12 trans-10 (CLA-t10) 0.17 0.66 0.15 0.18 0.51 0.56 0.10 0.82
 C18:2 cis-9 trans-11 (RA) 0.49 0.37 0.05 0.21 0.44 0.69 0.11 0.27
 C18:2 cis-9 cis-12 (LA) 6.42 8.07 0.40 0.02 15.1 17.6 0.93 0.19
 C18:3 cis-6 cis-9 cis-12 0.15 0.27 0.04 0.11 0.49 0.57 0.09 0.67
 C18:3 cis-9 cis-12 cis-15 (LNA) 1.47 2.28 0.16 < 0.01 1.05 1.73 0.19 0.05
 C20:2 0.43 0.37 0.06 0.63 0.35 0.44 0.10 0.68
 C20:3 n-6 0.63 0.65 0.07 0.89 4.04 5.11 0.38 0.17
 C20:4 n-6 nd. nd. nd. nd. 0.51 0.42 0.05 0.44
 C20:5 n-3 nd. nd. nd. nd. 0.74 0.64 0.14 0.73
 C22:2 0.08 0.16 0.04 0.28 0.62 0.49 0.15 0.72
 C22:5 n-3 nd. nd. nd. nd. 0.64 0.90 0.09 0.17
 C22:6 n-3 nd. nd. nd. nd. 0.45 0.79 0.15 0.27
∑ SFA 70.5 67.3 0.96 0.10 40.8 34.9 1.24 0.01
∑ UFA 29.5 32.7 0.96 0.10 59.2 65.1 1.24 0.01
∑ MUFA 18.7 20.1 0.60 0.66 34.3 35.1 0.95 0.70
∑ PUFA 10.8 12.5 0.51 < 0.01 24.9 30.0 1.01 0.07
∑ n-6 8.90 9.72 0.42 0.02 21.6 25.2 1.23 0.17
∑ n-3 1.47 2.28 0.16 < 0.01 2.88 4.06 0.31 0.07
n-6/n-3 6.05 4.37 0.39 0.02 7.86 6.74 0.73 0.47
PUFA/SFA 0.15 0.19 0.01 0.07 0.62 0.86 0.05 0.01
LNA/LA 0.21 0.30 0.02 0.02 0.07 0.10 0.01 0.31
∑ C18:1 15.7 16.8 0.64 0.45 27.4 26.8 1.22 0.81
∑ C18:1 trans- 5.48 5.08 0.28 0.52 1.37 0.96 0.11 0.06
∑ C18:1 cis- 10.2 11.7 0.58 0.23 26.1 25.8 1.19 0.94
∑ CLA 0.64 0.99 0.19 0.36 0.95 1.61 0.18 0.04
∑ MCFA 23.3 26.3 0.94 0.12 26.3 22.8 1.25 0.17
∑ LCFA 76.7 73.7 0.94 0.12 73.7 77.2 1.25 0.17
BH intermediates 7.81 7.76 0.46 0.95 nd. nd. nd. nd.
LA BH, % 74.2 67.6 1.58 0.03 nd. nd. nd. nd.
LNA BH, % 94.4 91.5 0.61 < 0.01 nd. nd. nd. nd.
RA/VA 0.08 0.11 0.02 0.50 nd. nd. nd. nd.
Desaturation index (DI) ∆9 nd. nd. nd. nd. 0.41 0.47 0.01 0.01
D∆9. C14:1/C14:0 nd. nd. nd. nd. 0.29 0.37 0.06 0.50
D∆9. 16:1/16:0 nd. nd. nd. nd. 0.07 0.07 0.01 0.85
D∆9. 18:1/18:0 nd. nd. nd. nd. 0.59 0.65 0.01 0.01
D∆9. RA/VA nd. nd. nd. nd. 0.27 0.48 0.06 0.04
D∆9. MUFA/SFA nd. nd. nd. nd. 0.46 0.50 0.01 0.08
D∆5. n-6. 20:4n-6/20:3n-6 nd. nd. nd. nd. 0.12 0.08 0.01 0.11
D∆5. D6. n-6. 20:4n-6/18:3n-6 nd. nd. nd. nd. 0.53 0.43 0.06 0.43
D∆4. n-3. 22:6n-3/22:5n-3 nd. nd. nd. nd. 0.33 0.41 0.06 0.53
Elongase index nd. nd. nd. nd. 0.71 0.72 0.01 0.92
Thrombogenic index nd. nd. nd. nd. 0.85 0.62 0.05 < 0.01
Atherogenicity index nd. nd. nd. nd. 0.63 0.48 0.03 0.03
  1. CON control diet, CAL Coleus amboinicus Lour. diet, SEM standard error of means, SFA saturated fatty acids, UFA unsaturated fatty acids, MUFA monounsaturated fatty acids, PUFA polyunsaturated fatty acids, BH biohydrogenation, LNA linolenic acid, RA rumenic acid, VA vaccenic acid, LA linoleic acid, MCFA medium-chain fatty acids, LCFA long-chain fatty acids, nd. not determined
  2. Means in the same row indicate significant differences at P < 0.05 and tended to significant at P < 0.10