Fig. 5

A sPLS-DA analysis for gestation duration deviation showing sample distribution in component 1 and component 2. Seminal plasma samples from entire ejaculates (24 ejaculates) of eight artificial insemination-boars (three ejaculates per boar) were used. Fertility parameters were recorded from 1,525 inseminations. The colored areas represent the 95% confidence interval. Boars with longer gestation duration deviation (ranging from 0.10 to 0.52; n = 4) are represented in red and those with shorter gestation duration deviation (ranging from − 0.85 to 0.03; n = 4) are shown in blue. Each dot symbolizes an ejaculate. B Loading plot for components 1 and 2 for the sPLS-DA model. Variables are ranked by the absolute values of their loadings. C Receiver operating characteristic (ROC) curve analysis for malonate and fumarate and pregnancy duration. They show the ability of metabolites to discriminate the number of stillbirths per litter after artificial insemination with semen doses. AUC: area under the curve; CI: confidence interval