Fig. 4

A sPLS-DA analysis for the number stillbirths per litter deviation showing sample distribution in component 1 and component 2. Seminal plasma samples from entire ejaculates (24 ejaculates) of eight artificial insemination-boars (three ejaculates per boar) were used. Fertility parameters were recorded from 1,525 inseminations. The colored areas represent the 95% confidence interval. Boars with decreased stillbirths per litter deviation (ranging from − 0.10 to 0.02; n = 4) are represented in blue and those with increased stillbirths per litter deviation (ranging from 0.05 to 0.14; n = 4) are shown in red. Each dot symbolizes an ejaculate. B Loading plot for components 1 and 2 for the sPLS-DA model. Variables are ranked by the absolute values of their loadings. C Receiver operating characteristic (ROC) curve analysis for citrate, creatine, phenylalanine, tyrosine and malonate and stillbirths per litter. They show the ability of metabolites to discriminate the number of stillbirths per litter after artificial insemination with semen doses. AUC: area under the curve; CI: confidence interval