Fig. 3

A sPLS-DA analysis for litter size deviation showing sample distribution in component 1 and component 2. Seminal plasma samples from entire ejaculates (24 ejaculates) of eight artificial insemination-boars (three ejaculates per boar) were used. Fertility parameters were recorded from 1525 inseminations. The colored areas represent the 95% confidence interval. Boars with decreased litter size deviation (ranging from − 0.40 to 0.02; n = 4) are represented in blue and those with increased litter size deviation (ranging from 0.11 to 0.52; n = 4) are shown in red. Each dot symbolizes an ejaculate. B Loading plot for components 1 and 2 for the sPLS-DA model. Variables are ranked by the absolute values of their loadings. C Receiver operating characteristic (ROC) curve analysis for carnitine, glucose, sn-glycero-3-phosphocholine, glutamate and hypotaurine concentrations in seminal plasma and litter size deviation. Seminal plasma samples from entire ejaculates (24 ejaculates) of eight artificial insemination-boars (three ejaculates per boar) were used. Fertility parameters were recorded from 1,525 inseminations. The plot shows the ability of the metabolites to discriminate litter size of semen doses. AUC: area under the curve; CI: confidence interval