Fig. 6
Effect of HS and supplemental 25-OH-D3 in broilers at d 39 on markers of bone metabolism. A Tartrate-resistant acid phosphatase (TRAP) staining of tibia sections. B Serum bone resorption biomarker C-terminal cross-linked telopeptide of type I collagen (CTx) concentrations were determined. C mRNA expression of bone remolding in the tibia proximal end were quantified. D Serum bone formation biomarker alkaline phosphatase activity (ALP) were analyzed. Treatments Ctrl, PF and HS without common superscript are significantly different at P < 0.05. n.s., denotes no significant difference between HS and HS + 25-OH-D3 at P < 0.05. RANKL: Receptor activator of nuclear Factor-κ B ligand; OPG: Osteoprotegerin; V-ATPase: Vacuolar-type H+-ATPase; VDR: Vitamin D receptor; Runx2: Runt-related transcription factor 2; Dmp1: Dentin matrix protein1; Phex: Phosphate regulating endopeptidase homolog x-linked