Fig. 2

NORHA is a novel cytoplasmic lncRNA involved in follicular atresia in pigs. a Flow diagram showing the location and predicted length (XR_304632, 7,082 bp, yellow line) of LOC102167901 in the GenBank database. According to reference sequences, we designed a special primer to isolate full-length LOC102167901 (a novel lncRNA, 1,566 bp, shown by the red line) through a RACE assay (Fig. S4a). b, c The coding potential of novel transcripts was predicted by the CPAT (b) and CPC (c) methods. LOC102167901 is the sequence obtained from the NCBI database. MALAT1, H19 and GAS5 are well-known lncRNAs, and GAPDH, SMAD4 and YY1 are protein-coding genes. d Tissue expression profile of NORHA. e Subcellular distribution of NORHA in GCs. The expression levels of NORHA, GAPDH and U6 (reference genes) in the nucleus (NORHA and U6) and cytoplasm (NORHA and GAPDH) were quantified by qRT-PCR. f The correlation of follicular NORHA levels and the P4/E2 ratio in follicular fluid