Table 3 qPCR primer sequence
From: Dietary calcium supplementation promotes the accumulation of intramuscular fat
Gene | Sequence(5'→3') |
|---|---|
MyOG | F: AGGAAGTCTGTGTCGGTGGA |
R: AGGCGCTCAATGTACTGGAT | |
MyHC | F: GAATGGCAAGACGGTGACTGTG |
R: GGAAGCGTAGCGCTCCTTGAG | |
MEF2C | F: ACCAGGACAAGGAATGGGAG |
R: GGCGGCATGTTATGTAGGTG | |
TNNI1 | F: ATGCCGGAAGTTGAGAGGAA |
R: CTGAAGGGCACTGAGAGACA | |
PGC-1α | F: AGCCTCTTTGCCCAGATCTT |
R: GGCAATCCGTCTTCATCCAC | |
TNNC1 | F: GCAAGGTGATGAGGATGCTG |
R: GACTTCCCTTTGCTGTCGTC | |
MYH1 | F: GGACCCACGGTCGAAGTTG |
R: CCCGAAAACGGCCATCT | |
MYH4 | F: CAATCAGGAACCTTCGGAACAC |
R: GTCCTGGCCTCTGAGAGCAT | |
TNNC2 | F: CGGCTCCATCGACTTTGAAG |
R: AGCAGCTCATCGATCTCCTC | |
TNNI2 | F: GATGAGGAGAAGCGCAACAG |
R: TTTCTCCTCTTCAGCCACGT | |
β-actin | F: CAGCCTTCCTTCTTGGGTAT |
R: TGGCATAGAGGTCTTTACGG |