Fig. 2
From: The circular RNA circCPE regulates myoblast development by sponging miR-138
Overexpression of circCPE promotes the proliferation of Myoblasts. (A) circCPE was successfully overexpress but had no effect on the linearCPE mRNA. (B and C) The expression of CDK2, PCNA, Cyclin D1, and Cyclin E were detected by real-time qPCR and western blots after transfection with pcD2.1-circCPE vector. (D and E) EdU (Scale bars, 2000 μm) and CCK-8 assays were used to measure the ability of proliferation. (F) The cell cycle distribution was evaluated using a flow cytometry assay. Values are means ± SEM for three individuals. P < 0.05, P < 0.01