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Table 2 Gas production and rumen fermentation parameters by endolysin LyJH307 supplementation in an in vitro experiment at 12 h of incubation

From: Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism

 

Treatments1

  

Itemsa

CON

LyJH307

SEM

P-value

Gas2, mL/g DM

 6 h

52.2

50.0

1.10

0.12

 9 h

87.3

82.7

1.91

0.11

 12 h

106

103

2.2

0.25

IVDMD, %

40.1

38.7

1.16

0.36

NH3-N, mg/100 mL

7.87

9.21

0.553

0.15

pH

5.57

5.69

0.033

0.034

Total VFA, mmol/L

83.1

89.1

4.39

0.26

Acetate, mmol/mol

428

448

6.4

0.036

Propionate, mmol/mol

342

325

4.4

0.038

Butyrate, mmol/mol

200

195

3.0

0.24

Iso-butyrate, mmol/mol

1.96

2.16

0.171

0.35

Valerate, mmol/mol

22.2

20.9

0.80

0.28

Iso-valerate, mmol/mol

6.96

8.12

1.726

0.63

A:P ratio

1.25

1.38

0.033

0.029

Total lactate, mmol/L

16.8

18.9

0.87

0.082

D-lactate, mmol/L

9.74

11.26

0.404

0.034

L-lactate, mmol/L

7.02

7.63

0.642

0.46

  1. a SEM Standard error of the mean, DM Dry matter, IVDMD In vitro dry matter digestibility, NH3-N Ammonia-nitrogen, VFA Volatile fatty acids, A:P ratio Acetate to propionate ratio
  2. 1 CON, corn grain with elution buffer of the same volume of endolysin treatment; LyJH307, corn grain with endolysin LyJH307 (4 U/mL)
  3. 2 Treatment effect, P = 0.0114; Time effect, P < 0.0001; Interaction, P = 0.9353
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891