Fig. 8

Cysteamine (CS) pretreatment attenuates the effects of H₂O₂ on angiogenesis. A, E The levels of ROS. PVECs were pretreated with various concentrations of CS (0.5, 1 or 2 mmol/L) for 2 h and then treated with 200 μmol/L H₂O₂ for 24 h (n = 6; bar = 100 μm). B, F Scratch healing assay of migratory distance. PVECs were pretreated with various concentrations of CS (0.5, 1 or 2 mmol/L) for 2 h and then treated with 200 μmol/L H₂O₂ for 24 h (n = 3; bar = 500 μm). C, G Trans-well migration assay of the migratory number of PVECs. After different treatments as described above, PVECs were added to the upper chamber of a trans-well and incubated for 48 h, followed by quantifying PVECs that invaded through the chamber (n = 3; bar = 500 μm). D, H Representative images of tube formation of PVECs after different treatments as described above (n = 5; bar = 100 μm). I CCK8 assay was used to measure cell viability after different treatments as described above (n = 6). Data are presented as mean ± SEM (n = 3). Different letters indicate significant differences at P < 0.05