Fig. 5

Effects of the miR-34b mimic and inhibitor on the Akt/mTOR signaling pathway in BMECs. a-d The relative mRNA expression level of Akt, mTOR, 4E-BP1, and P70S6K. e The total protein and phosphorylated protein expression level of Akt, mTOR, 4E-BP1, and P70S6K. Values are presented as the mean ± SEM. *, P < 0.05; **, P < 0.01. BMECs = bovine mammary epithelial cells, NC = negative control