Fig. 2
Effects of PQQ on oxidative cytokines and tight junction proteins in cells cultured with H2O2. a-c, Levels of SOD, GSH-Px and MDA in IPEC-J2 cells. d, Western blot analysis of ZO-1, ZO-2, ZO-3, occludin, and claudin-1. Densitometric values were normalized to those of Tubulin. e, Statistical analysis of the data in D. CTRL, cells cultured in basal medium; H2O2, cells cultured with 200 μmol/L H2O2; PQQ, cells cultured with 10 nmol/L PQQ for 6 h and then incubated with both 200 μmol/L H2O2 and 10 nmol/L PQQ for another 2 h. * denotes a significant difference (P < 0.05) with respect to the CTRL group. # denotes a significant difference (P < 0.05) with respect to the H2O2 group. n = 3