Fig. 2

The effect of PERK inhibitor (GSK) on cell viability and apoptosis in IPEC-J2 cells. Cells were pre-treated with an apoptosis inhibitor (GSK, 1 or 10 μmol/L) for 24 h, followed by challenge with RV (10 MOI) for 1 h, and then incubated with DMEM/F12 for a further 24 h. a Cell viability was measured by CCK8 assay. b Apoptotic populations of cells double stained with PI- and FITC-labeled Annexin V were measured by flow cytometry. c The effect of RV infection on the mRNA expressions of apoptosis related genes in IPEC-J2 cells. ^*P < 0.05, ^**P < 0.01, data are expressed as means ± S.D. from three independent experiments at least