Fig. 2

Hematoxylin and eosin staining, Se concentrations, and spleen index in the Se-adequate group and Se-deficient group spleens. Se deficiency caused blurring of the boundary between red and white pulp, and decreased the white pulp volume and cell density (yellow arrow) in the spleen, particularly narrowing of the periarterial lymphatic sheath (blue arrow). In addition, Se deficiency significantly decreased the density and number of splenic cord cells (red arrow), thereby increasing the splenic sinusoid space and the splenic red pulp volume. Photomicrographs are shown at 100× and 200× magnification