Fig. 1

MYOD1 amino acid sequence analysis. The MYOD1 amino acid sequences of the pig (NP_001002824.1), mouse (NP_034996.2), human (NP_002469.2), horse (NP_001304182.1), cow (NP_001035568.2), and sheep (NP_001009390.1) were acquired from the NCBI database. a Schematic representation of the MYOD1 protein. The whole protein (black line) and its acidic and H/C domains (light gray boxes), basic domain (dark gray box), helix motifs (slashed boxes), and loop motif (black box) are indicated. The diagram is scaled. b Alignment and comparison of the MYOD1 amino acid sequence via the multiple sequence alignment program MUSCLE. The acidic domain (black line), H/C domain (light gray line), basic domain (gray box), helix1 (red box) -loop (blue box) -helix2 (green box), and helix3 motif (dark gray line) are indicated in the amino acid alignment. Arg¹¹¹ and Ala¹¹⁴-Thr¹¹⁵ are marked in red. The consensus sequence of amino acids among the species is described with an asterisk at the bottom line