Fig. 3

The inhibitory effect of MYOD1 on adipocyte differentiation was achieved by its downstream gene miR-206. a Over-expression of MYOD1 up-regulated miR-1, miR-133a, miR-133b, and miR-206 expression in ICPs. b MYOD1 knockout down-regulated miR-1, miR-133a, miR-133b, and miR-206 expression in ICPs. c MYOD1 over-expression promoted the relative luciferase activity of the pGL4.10_-1234 bp and pGL4.10_-1876 bp reporter in ICPs. d Relative miR-206 expression during ICPs differentiation. e Transfected with miR-206 mimic significantly promoted miR-206 expression in ICPs. f Over-expression of miR-206 reduced the lipid droplet formation by oil red O staining on day 3. g mRNA levels of adipocyte marker genes were analyzed with qPCR. h Transfected with miR-206 inhibitor significantly reduced miR-206 expression in ICPs. i Inhibition of miR-206 promoted the lipid droplet formation by oil red O staining on day 3. j mRNA levels of adipocyte marker genes were analyzed with qPCR. k Inhibition of miR-206 could counteract the inhibition effect of MYOD1 over-expression on adipocyte differentiation. l mRNA levels of adipocyte marker genes were analyzed with qPCR. Data are shown as mean ± SD of three biological replicates. The t-test was used to analyze the statistical differences between groups. *, P < 0.05