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Table 2 Muscle physiochemical parameters of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks1

From: Dietary isoleucine improved flesh quality, muscle antioxidant capacity, and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂)

Items2 Dietary Ile levels, g/kg SEM P-values
5.0 7.5 10.0 12.5 15.0 17.5 20.0 Linear Quadratic
pH45min 7.11a 7.15a 7.50ab 7.62b 7.60b 7.59b 7.50ab 0.05 <  0.0001 < 0.0001
pH24h 6.83 6.83 6.78 6.78 6.89 6.83 6.78 0.03 0.79 0.76
Shear force 0.26abc 0.22a 0.19a 0.32c 0.25abc 0.32bc 0.22ab 0.02 0.06 0.11
Cooking loss 20.49 18.27 19.78 19.28 19.42 17.45 19.25 0.72 0.16 0.56
Lactate 0.27c 0.24bc 0.21abc 0.16a 0.17ab 0.19ab 0.18ab 0.01 <  0.0001 <  0.01
Cathepsin B 2.04abc 1.72ab 1.63a 2.16abc 2.44c 3.34d 2.38bc 0.08 <  0.0001 0.12
Cathepsin L 0.87c 0.88c 1.05d 0.80bc 0.74b 0.72b 0.61a 0.01 <  0.0001 <  0.0001
Hydroxyproline 0.15ab 0.22bc 0.26c 0.49d 0.30c 0.13ab 0.12a 0.01 <  0.01 <  0.0001
  1. 1 Values are mean ± SEM of three replicate groups with 6 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)
  2. 2 Shear force, N/(mm·s); Cooking loss, %; Lactate content, mmol/g protein; Cathepsin B and L activities, U/g protein; Hydroxyproline concentration, μg/mg protein