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Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cells

Fig. 7

Relative gene expression analysis after knockdown of predicted transcription factors (TFs) in cultured primordial germ cells (PGCs) and embryonic stem cells (ESCs). a Efficiency of small interfering RNA (siRNA)-mediated knockdown of TP53 in PGCs was analyzed by quantitative reverse-transcription PCR (RT-qPCR). Relative expression of NANOG was determined. b–f Efficiency of siRNA-mediated knockdown of CEBPA, CEBPB, CEBPD, CEBPG, and CEBPZ in ESCs was analyzed by RT-qPCR. Relative expression of NANOG was determined in each sample. Error bars indicate the standard deviation of triplicate analyses. Significant differences are indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891