Table 2 Primers used for real-time PCR analysisa
Gene nameb | Primer sequence (5′ to 3′) | Accession number |
|---|---|---|
GAPDH | F:AGAACATCATCCCAGCGTCC | NM_204305 |
R:CGGCAGGTCAGGTCAACAAC | ||
MHC-II | F:CCACGGACGTGATGCAGAAC | 113,206,149 |
R:ACCGCGCAGGAACACGAAGA | ||
Mucin 2 | F:TTCATGATGCCTGCTCTTGTG | XM_421035 |
R:CCTGAGCCTTGGTACATTCTTGT | ||
Lyz C | F:GACGATGTGAGCTGGCAG | NM_205281 |
R:GGATGTTGCACAGGTTCC | ||
pIgR | F:ATTTGTCACCACCACAGCCA | NM_001044644 |
R:GAGTAGGCGAGGTCAGCATC | ||
IgA | F:ACCACGGCTCTGACTGTACC | S40610.1 |
R:CGATGGTCTCCTTCACATCA | ||
NF-κB | F:TGGAGAAGGCTATGCAGCTT | NM_205134.1 |
R:CATCCTGGACAGCAGTGAGA | ||
IL-2 | F:AGTGCACCCAGCAAACTCTG | NM_204153.1 |
R:TCCGGTGTGATTTAGACCCGT | ||
IL-4 | F:GCTCTCAGTGCCGCTGATG | NM_0010079.1 |
R:GAAACCTCTCCCTGGATGTCAT | ||
IL-6 | F:GATCCGGCAGATGGTGATAA | NM_204628.1 |
R:AGGATGAGGTGCATGGTGAT | ||
IFN-γ | F:AAAGCCGCACATCAAACACA | NM_205149.1 |
R:GCCATCAGGAAGGTTGTTTTTC | ||
TGF-β1 | F:GCCGACACGCAGTACACCAAG | NM_001318456.1 |
R:GCAGGCACGGACCACCATATTG | ||
CD3d | F:TGTTGTCGCCACTGTCTTGCTG | NM_205512.1 |
R:GTCCATCATTCCGCTCACCAAGG | ||
CD4 | F:GATGGAGAGGTGTGGAGCAG | NM_204649 |
R:CCTCCTTTCCTGCAATCCCA | ||
TCR β subunit | F:ACTGGTATGCCTGGCCTCTGG | M81149.1 |
R:CCACTCCTTCTGTCCTCTTCACAC | ||
TCR ζ subunit | F:AGCTTAGCCAGGCCTCTGAA | AJ002317.1 |
R:GGTGCCCAGCACATCGTATT |