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Table 2 Total amino acids, agmatine, and polyamines in uterine flushings (μg)

From: Pre-implantation exogenous progesterone and pregnancy in sheep: I. polyamines, nutrient transport, and progestamedins

 

Effect

 

Day 9

Day 12

Nutrient

CO

P4

CO

P4

Amino Acid

 Alanine

5 ± 1.5a

7.2 ± 1.9a

8.6 ± 2.2a

17.8 ± 1.4b

 β-Alanine

0.2 ± 0.04

0.4 ± 0.2

0.4 ± 0.1

0.5 ± 0.2

 Arginine

2.5 ± 1.3

4.1 ± 1.8

7.2 ± 2.2

8.3 ± 3.8

 Asparagine

1.5 ± 0.5a

1.8 ± 0.6ab

4.2 ± 1ab

5.2 ± 0.6b

 Aspartate

2.2 ± 0.5ab

1.6 ± 0.3a

4.1 ± 0.8b

4.2 ± 0.5ab

 Citrulline

0.7 ± 0.2

3.4 ± 1.6

3.7 ± 1.1

1.8 ± 1.2

 Glutamate

17.5 ± 3.6ab

8.8 ± 1.3a

23.8 ± 3.5b

29.1 ± 3.5b

 Glutamine

3.1 ± 1

3.7 ± 1.5

11.5 ± 2.7

12.1 ± 2.3

 Glycine

65.6 ± 21.4

81.5 ± 16.4

76.6 ± 14.8

59 ± 4.8

 Histidine

0.8 ± 0.4

1.4 ± 0.6

2.1 ± 0.5

2.4 ± 0.9

 Isoleucine

2 ± 1.8ab

1.5 ± 0.2a

1.5 ± 0.2a

3.1 ± 0.4b

 Leucine

6 ± 1.8

3.7 ± 1.1

6.6 ± 1.2

9.1 ± 1.2

 Lysine

6 ± 2

2.8 ± 0.6

5.2 ± 1.5

10.9 ± 2.7

 Methionine

1.4 ± 0.6

1.1 ± 0.5

2 ± 0.6

3.4 ± 1

 Ornithine

0.2 ± 0.06a

0.4 ± 0.1ab

0.5 ± 0.1ab

0.9 ± 0.2b

 Phenylalanine

2.7 ± 0.9

1.9 ± 0.5

1.9 ± 0.4

3.5 ± 1

 Serine

12 ± 3.6a

41.1 ± 12.2b

41.4 ± 10.1b

52.9 ± 13.1b

 Taurine

20 ± 8.3

18.3 ± 4.4

23 ± 5.7

25.5 ± 0.7

 Threonine

3.4 ± 1.4

6.7 ± 1.7

13.4 ± 3.8

12.4 ± 3.9

 Tryptophan

0.7 ± 0.4

1.3 ± 0.4

1.6 ± 0.3

1.4 ± 0.7

 Tyrosine

1.7 ± 0.8

1.3 ± 0.4

1.2 ± 0.2

2.9 ± 1.3

 Valine

10 ± 3.4

5.6 ± 1.4

6.1 ± 1.1

5.8 ± 0.6

Agmatine and polyamines

 Agmatine

0.1 ± 0.04

0.04 ± 0.01

0.08 ± 0.02

0.02 ± 0.0001

 Putrescine

1.1 ± 0.5

0.8 ± 0.4

1.1 ± 0.3

0.1 ± 0.03

 Spermidine

0.9 ± 0.4

0.8 ± 0.4

1.3 ± 0.3

0.1 ± 0.03

 Spermine

1.2 ± 0.6

2 ± 1.2

2.3 ± 0.7

1.1 ± 0.8

  1. Values represent means ± SEM
  2. Different superscripts within Effect columns are significantly different (P < 0.05)
  3. Only uterine flushings from ewes which were considered pregnant with normally developed conceptuses were utilized for these analyses