Fig. 2

CRTC3 overexpression increases lipid accumulation in white adipocytes. The GFP control (CON) and CRTC3 overexpressing (OE) cells were used for the examination at 48 h after treatment. a. Image of immunofluorescence staining for CRTC3 (green) and perilipin 1 (red) in CON or OE adenovirus-treated subcutaneous adipocytes. Nuclei were stained with DAPI (blue). Scale bar, 20 μm. b. Protein levels of CRTC3 and perilipin in CON or OE adipocytes. c. The relative mRNA levels of CRTC3 in porcine subcutaneous adipocytes. d. TG contents in CON or OE adipocytes. e. Relative expression of adipose deposition-related gene. f. Relative expression of lipolysis-related genes. Relative expression of the target genes in every sample obtained using real-time PCR was normalized to 18S rRNA expression. The data represent the fold change in OE cells relative to the CON cells, which was arbitrarily defined as 1. n = 6. SEM: standard error of the mean. * P < 0.05; **P < 0.01