Fig. 8

Load of Salmonella Typhimurium in faeces affected by sampling time-points and mean percent value of Salmonella Typhimurium in organs at week 12 post-challenge. aSalmonella load in log₁₀ mMPN per gram of faeces at different sampling time-points in three treatment groups (SX- Salmonella Typhimurium challenged; CPX- Salmonella Typhimurium challenged and continuously supplemented feed with probiotic; IPX- Salmonella Typhimurium challenged and continuously supplemented feed with probiotic). bSalmonella load in log₁₀ mMPN per gram of faeces affected by treatment groups. Mean percent value of Salmonella Typhimurium in c cecum; d jejunum; e liver; f spleen; g magnum/infundibulum and h shell gland. For Salmonella load in the organs, small pieces of the tissues with known weight were homogenised in 500 μL PBS and a 100-μL was plated on XLD media. From the same tissue homogenates, a 100-μL was enriched in BPW followed by RVS and streaked on XLD/BSA media. The XLD/BSA plates were read as positive (1) and negative (0). The data were analysed in Statview software for getting mean percent values that represent the load of Salmonella Typhimurium in organs