Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model

Table 2 Effects of in ovo feeding of L-arginine on growth performance of layer chicks

Item³			Arg dosage (mg/egg)			Pooled SEM	P-value
Item³	NC¹	SC²	2	6	10	Pooled SEM	ANOVA	Linear	Quadratic
d 0 to 14
ADG, g	6.14	6.40	6.35	6.29	6.56	0.07	0.451	0.757	0.568
ADFI, g	11.45	12.11	11.55	11.60	11.85	0.12	0.410	0.109	0.279
F/G	1.87	1.90	1.82	1.82	1.87	0.02	0.500	0.442	0.714
d 15 to 21
ADG, g	7.86^b	7.62^b	7.62^b	7.70^b	8.56^a	0.10	0.011	0.021	0.032
ADFI, g	19.42^b	19.62^b	19.19^b	19.72^b	20.82^a	0.16	0.019	0.037	0.044
F/G	2.53	2.58	2.49	2.50	2.41	0.02	0.213	0.622	0.535
d 0 to 21
ADG, g	6.45^b	6.71^ab	6.69^ab	6.72^ab	7.00^a	0.06	0.030	0.073	0.143
ADFI, g	13.80^b	14.61^a	14.25^ab	14.02^ab	14.66^a	0.11	0.021	0.847	0.075
F/G	2.14^a	2.14^a	2.13^a	2.08^b	2.07^b	0.01	0.016	0.047	0.032
d 22 to 42
ADG, g	12.23	12.65	12.55	12.86	12.52	0.09	0.228	0.869	0.647
ADFI, g	32.30	32.37	32.80	33.49	33.00	0.22	0.075	0.545	0.284
F/G	2.62	2.61	2.62	2.61	2.64	0.01	0.957	0.581	0.833
d 0 to 42
ADG, g	9.28	9.55	9.51	9.55	9.58	0.05	0.400	0.509	0.826
ADFI, g	22.49	22.67	22.25	23.16	23.05	0.15	0.291	0.395	0.427
F/G	2.45	2.42	2.40	2.41	2.41	0.01	0.564	0.717	0.935

^a–bMeans within a row with no common superscripts differ significantly (P < 0.05). Data are the mean of 8 replicates. ¹NC, non-injected control group; ²SC, saline-injected control group. ³ADG, average daily gain; ADFI, average daily feed intake; F/G, feed conversion ratio (feed: gain, g: g). Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing concentrations of L-arginine solution

ISSN: 2049-1891