Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model

Table 1 Effects of in ovo feeding of L-arginine on hatchability, body weight of layer chicks

Item			Arg dosage (mg/egg)			Pooled SEM	P-value
Item	NC¹	SC²	2	6	10	Pooled SEM	ANOVA	Linear	Quadratic
Hatchability, %	91.36	92.72	93.67	91.91	91.00	0.67	0.518	0.910	0.777
HW³, g	42.50	42.34	43.26	42.30	42.19	0.15	0.137	0.297	0.379
BW⁴, g
d 7	73.38	74.67	75.94	74.37	74.97	0.35	0.220	0.665	0.827
d 14	122.42	125.89	126.02	124.35	126.95	0.82	0.474	0.529	0.795
d 21	174.60^b	177.81^ab	178.56^ab	181.00^ab	183.85^a	1.03	0.039	0.018	0.382
d 42	434.29^b	444.65^ab	444.72^ab	452.60^a	452.28^a	2.21	0.042	0.202	0.301

^a–bMeans within a row with no common superscripts differ significantly (P < 0.05). ¹NC, non-injected control group; ²SC, saline (0.85% NaCl)-injected control group. ³HW, hatching weight; ⁴BW, body weight. Data are the mean of 8 replicates. Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing concentrations of L-arginine solution

ISSN: 2049-1891