Fig. 2

Confirmation of the natural products that induce mRNA expression of multiple porcine HDP genes in IPEC-J2 (a) and 3D4/31 cells (b). Cells were treated with or without different concentrations (5, 20, or 80 μmol/L) of the hits for 24 h, followed by RT-qPCR analysis of four representative porcine HDP genes (pBD3, pBD2, PG1-5, and pEP2C). The results are the mean ± SEM of three independent experiments. *P < 0.05 vs. non-treated control by unpaired Student’s t-test