Effects of brewers’ spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

Table 5 Effect of brewers’ spent grain (BSG) protein hydrolysates or antibiotics on microbial N synthesis and microbial community of FPA in RUSITEC

Item	Treatments^d				SEM	P <
Item	Con	AlcH	FlaH	Ant	SEM	P <
Production of microbial N^e, mg/d
LAB^f	60.7^a	60.7^a	53.3^b	48.2^c	1.94	0.05
FPB^g	7.0	6.2	6.4	7.0	1.04	0.51
FPA^h	13.7^c	16.6^b	17.6^ab	19.4^a	1.82	0.01
Total	80.3^a	82.5^a	76.2^b	73.5^b	1.83	0.05
Efficiency of microbial proteinⁱ	7.0	7.1	6.9	6.9	0.26	0.45

^{a, b, c} Least square means within a row with different superscripts differ (P < 0.05)
^d Con Control, no antioxidant peptide and no antibiotics, AlcH Alcalase hydrolysates, 10 mg per gram of TMR (DM basis), FlaH Flavourzyme hydrolysates, 10 mg per gram of TMR (DM basis), Ant antibiotics, 0.8 mg monensin + 1 mg tylosin per gram of TMR (DM basis)
^e Samples from d 14 to 15
^f Liquid associate bacteria
^g Feed particle-bound bacteria
^h Feed particle-associated bacteria
ⁱ Efficiency of microbial protein, mg microbial N production/g OM fermented

ISSN: 2049-1891