Fig. 2
Transfection with OPmiR-365-3p and pcDNA.3.1 for 24 h in PBMs for detecting effects on cell proliferation. a The expression of CDK2, PCNA and CCND1 at the mRNA level and protein level by qRT-PCR and western blotting, respectively. The OP group denotes the group treated with the vector OPmiR-365-3p, which overexpresses bta-miR-365-3p. b The PBMs transfected with empty plasmid pcDNA3.1 by flow cytometer assay. c The PBMs transfected with OPmiR-365-3p by flow cytometer assay. d Statistics of the counts of G1, G2 and S stage cells of flow cytometer assay. Error bars indicates the SD among three repeats. e EdU cell proliferation index, EdU (red), DAPI (blue), scale bars 200 μm. f Statistics of the percentage of dual positive cells. Error bars indicates the SD among three repeats. Values are mean ± SD for three biological replicates. Note: CDK2: cyclin-dependent kinase 2; PCNA: Proliferating cell nuclear antigen; CCND1: Cyclin D1 NC: Negative control transfected with the empty plasmid pcDNA3.1; OP: OPmiR_365-3p, the vector overexpressing bta-miR-365-3p; qRT-PCR: quantitative real-time polymerase chain reaction; SD: standard deviation. *P < 0.05, ** P < 0.01, *** P < 0.001