Fig. 6

The loss of RORγ binding decreases histone acetylation on CHO genes in TRF treated organoids. a RORγ directly binds on CHO gene, MVK, both in human and murine samples analyzed by ChIP-seq datasets. b ChIP-qPCR analysis of RORγ occupancy at the locus of MVK in organoids. c Schematic diagram depicting the locations of putative ROR element region of MVK gene and primers used for genomic DNA PCR. d TRF reduced RORγ enrichment on MVK gene promoter. e, f RORγ agonists SR0987 and Desmosterol e and overexpression (RORγ-1-TRF, RORγ-2-TRF) f restored the enrichment of RORγ at target loci of MVK. Data were presented as means ± SD of at least three independent experiments, *P < 0.05, **P < 0.01. using ANOVA with Tukey’s post hoc test