Fig. 3
TRF reduces cholesterol biosynthesis program in porcine liver organoids. a, b Total and free CHO contents in organoids (mg/μg protein). c Key genes expression (log2) of CHO biosynthesis normalized to control. d Western blotting analysis of CHO biosynthesis enzymes MVK, FDFT1, SQLE, EBP, DHCR24 selected based on mRNA expression (c), whereas GAPDH was used as an internal reference. Data were presented as means ± SD of at least three independent experiments, *P < 0.05, using two tailed Student's t-test